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  • Open Access


    Cloning of and analysis of cadmium resistant in Potentilla sericea


    BIOCELL, Vol.47, No.7, pp. 1571-1582, 2023, DOI:10.32604/biocell.2023.029106

    Abstract Background:Potentilla sericea is a heavy metal hyperaccumulator landscaping plant. MYB transcription factors play an important role in regulating plant stress response to adversity. However, there are few studies on MYB transcription factors in stress tolerance in Potentilla sericea. In this study, the gene was successfully cloned from Potentilla sericea. Methods: Bioinformatic analysis and real-time quantitative PCR (qPCR) methods were used to evaluate this gene. The transgenic A. thaliana were obtained by flower dipping and the gene function was identified by determining physiological indicators under cadmium stress. Results: The open reading frame of is 942 bp, which encodes 313 amino acids… More >

  • Open Access


    Functional Analysis of the Genotypic Differences in Response of Pea (Pisum sativum L.) to Calcareous-Induced Iron Deficiency

    Sameh Barhoumi1, Hasna Ellouzi2, Abdelmajid Krouma1,3,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 521-536, 2023, DOI:10.32604/phyton.2022.023555

    Abstract Lime-induced iron chlorosis is a major nutritional disorder causing severe plant growth and yield reduction in the calcareous soils of Tunisia. The understanding the behavior of key metabolic functions of peas on calcareous soils, the identification of useful traits of tolerance, and the exploration of the genotypic differences in response to this constraint remain the most efficient approaches due to their coast, environmental benefits, and sustainability. For this purpose, a greenhouse experiment was conducted on three pea genotypes (Alexandra: Alex, Douce de provence: DP, and Merveille de Kelvedon: MK) cultivated on calcareous soil (Fe-deficient) and fertile soil (control). Plant growth,… More >

  • Open Access


    Bioinformatics and Functional Analysis of High Oleic Acid-Related Gene GmSAM22 in Soybean [Glycine max (L.) Merr.]

    Shuo Qu1, Qi Cai2, Huimin Cui1, Lamboro Abraham1, Yaolei Jiao1, Guilong Ma2, Piwu Wang1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 501-519, 2023, DOI:10.32604/phyton.2022.023479

    Abstract High yield, high quality, stable yield, adaptability to growth period, and modern mechanization are the basic requirements for crops in the 21st century. Soybean oleic acid is a natural unsaturated fatty acid with strong antioxidant properties and stability. Known as a safe fatty acid, it has the ability to successfully prevent cardiovascular and cerebrovascular disorders. Improving the fatty acid composition of soybean seeds, can not only speed up the breeding process of high-quality high-oil and high-oleic soybeans, but also have important significance in human health, and provide the possibility for the development of soybean oil as a new energy source.… More >

  • Open Access


    Construction and Functional Analysis of CRISPR/Cas9 Vector of FAD2 Gene Family in Soybean

    Zhifeng Xiao, Yukun Jin, Qi Zhang, Abraham Lamboro, Baozhu Dong, Zhuoyu Yang, Piwu Wang*

    Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 349-361, 2022, DOI:10.32604/phyton.2022.017451

    Abstract Soybean oleic acid content is one of the important indexes to evaluate the quality of soybean oil. In the synthesis pathway of soybean fatty acids, the FAD2 gene family is the key gene that regulates the production of linoleic acid from soybean oleic acid. In this study, CRISPR/Cas9 gene editing technology was used to regulate FAD2 gene expression. Firstly, the CRISPR/Cas9 single knockout vectors GmFAD2-1B and GmFAD2-2C and double knockout vectors GmFAD2-2A-3 were constructed. Then, the three vectors were transferred into the recipient soybean variety Jinong 38 by Agrobacterium-mediated cotyledon node transformation, and the mutant plants were obtained. Functional analysis… More >

  • Open Access


    Heterologous expression of bacteriocin E-760 in Chlamydomonas reinhardtii and functional analysis

    Quezada-Rivera JJ1, RE Soria-Guerra2, FS Pérez-Juárez2, L Martínez-González2, SE Valdés- Rodríguez3, NL Vasco-Méndez1, JF Morales-Domínguez1

    Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 25-35, 2019, DOI:10.32604/phyton.2019.04549

    Abstract The use of antimicrobial peptides (AMPs) synthesized by bacteria (bacteriocins) is an alternative for combating multidrug resistant bacterial strains and their production by recombinant route is a viable option for their mass production. The bacteriocin E-760 isolated from the genus Enterococcus sp. has been shown to possess inhibitory activity against Gram-negative and Gram-positive bacteria. In this study, the expression of a chimeric protein coding for E-760 in the nucleus of C. reinhardtii was evaluated, as well as, its antibacterial activity. The synthetic gene E-760S was inserted into the genome of C. reinhardtii using Agrobacterium tumefaciens. A transgenic line was identified… More >

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