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Search Results (10)
  • Open Access

    ARTICLE

    Genome-Wide Identification of ABCC Gene Subfamily Members and Functional Analysis of CsABCC11 in Camellia sinensis

    Mingyuan Luo1, Shiyu Tian1, Xinzhuan Yao2, Yue Wan4, Zhouzhuoer Chen1, Zifan Yang4, Huagen Hao4, Fei Liu3, Hu Tang1,2,*, Litang Lu1,2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.8, pp. 2019-2036, 2024, DOI:10.32604/phyton.2024.052938 - 30 August 2024

    Abstract The ATP-binding cassette (ABC) transporter is a gene superfamily in plants. ATP-binding cassette subfamily C (ABCC) protein is a multidrug resistance-associated (MRP) transporter. They play various roles in plant growth, development, and secondary metabolite transport. However, there are few studies on ABCC transporters in tea plants. In this study, genome-wide association study (GWAS) analysis of epigallocatechin gallate (EGCG) content in 108 strains of Kingbird revealed that CsABCCs may be involved in EGCG transport. We identified 25 CsABCC genes at the genomic level of the tea plant, their phylogenetic tree, gene structure, targeted miRNA and other bioinformatics… More >

  • Open Access

    ARTICLE

    Cloning, Characterization and Transformation of Methyltransferase 2a Gene (Zmet2a) in Maize (Zea mays L.)

    Xin Qi1,#, Yu Wang1,#, Xing Zhang1, Xiaoshuang Wei1, Xinyang Liu1, Zhennan Wang1, Zhenhui Wang1,*, Fenglou Ling2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.7, pp. 1767-1779, 2024, DOI:10.32604/phyton.2024.052844 - 30 July 2024

    Abstract DNA methylation is an important epigenetic regulatory mechanism, it regulates gene expression by recruiting proteins involved in gene repression or by inhibiting the binding of transcription factor(s) to DNA. In this study, a novel methyltransferase 2a gene (Zmet2a) was cloned in maize and identified by polymerase chain reaction-base (PCR-base) using a bioinformatics strategy. The Zmet2a cDNA sequence is 2739 bp long and translates to 912 amino acid peptides. The Zmet2a protein revealed that it contains BAH and CHROMO structural domains, is a non-transmembrane protein that is hydrophilically unstable, and has no signal peptide structure. Meanwhile, we verified More >

  • Open Access

    ARTICLE

    The Identification of Phenylalanine Ammonia-Lyase (PAL) Genes from Pinus yunnanensis and an Analysis of Enzyme Activity in vitro

    Dejin Mu1,2, Lin Chen1,2, Heze Wang1,2, Zhaoliu Hu1,2, Sihui Chen1,2, Shi Chen1,2, Nianhui Cai1,2, Yulan Xu1,2, Junrong Tang1,2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 503-516, 2024, DOI:10.32604/phyton.2024.048786 - 28 March 2024

    Abstract Phenylalanine ammonia lyase (PAL) is the rate-limiting and pivotal enzyme of the general phenylpropanoid pathway, but few reports have been found on PAL genes in Pinus yunnanensis. In the present study, three PAL genes were cloned and identified from P. yunnanensis seedlings for the first time, namely, PyPAL-1, PyPAL-2, and PyPAL-3. Our results indicated that the open-reading frames of PyPAL genes were 2184, 2157, and 2385 bp. Phylogenetic tree analysis revealed that PyPALs have high homology with other known PAL genes in other plants. In vitro enzymatic analysis showed that all three PyPAL recombinant proteins could catalyze the deamination of L-phenylalanine to form More >

  • Open Access

    ARTICLE

    Transcriptome-Wide Identification and Functional Analysis of PgSQE08-01 Gene in Ginsenoside Biosynthesis in Panax ginseng C. A. Mey.

    Lei Zhu1,#, Lihe Hou1,3,#, Yu Zhang1, Yang Jiang1, Yi Wang1,2, Meiping Zhang1,2, Mingzhu Zhao1,2,*, Kangyu Wang1,2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 313-327, 2024, DOI:10.32604/phyton.2024.047938 - 27 February 2024

    Abstract Panax ginseng C. A. Mey. is an important plant species used in traditional Chinese medicine, whose primary active ingredient is a ginsenoside. Ginsenoside biosynthesis is not only regulated by transcription factors but also controlled by a variety of structural genes. Nonetheless, the molecular mechanism underlying ginsenoside biosynthesis has always been a topic in the discussion of ginseng secondary metabolites. Squalene epoxidase (SQE) is a key enzyme in the mevalonic acid pathway, which affects the biosynthesis of secondary metabolites such as terpenoid. Using ginseng transcriptome, expression, and ginsenoside content databases, this study employed bioinformatic methods to systematically… More >

  • Open Access

    ARTICLE

    Screen for autophagy-related biomarkers in osteoarthritis based on bioinformatic analysis

    CHAO LIU*

    BIOCELL, Vol.48, No.2, pp. 339-351, 2024, DOI:10.32604/biocell.2023.047044 - 23 February 2024

    Abstract Introduction: Osteoarthritis (OA) is still an important health problem, and understanding its pathological mechanisms is essential for its diagnosis and treatment. There is evidence that autophagy may play a role in OA progression, but the exact mechanism remains unclear. Methods: In this study, we adopted a multi-prong approach to systematically identify the key autophagy-related genes (ARGs) associated with OA. Through weighted gene co-expression network analysis, we initially identified significant gene modules associated with OA. Subsequent differential gene analysis performed on normal and OA specimens. Further analysis later using the MCC algorithm highlighted hub ARGs. These… More >

  • Open Access

    ARTICLE

    Cloning of and analysis of cadmium resistant in Potentilla sericea

    ZHENGHONG FENG1, BING GAO1, YU GAO2, JIANHUI WU1,*

    BIOCELL, Vol.47, No.7, pp. 1571-1582, 2023, DOI:10.32604/biocell.2023.029106 - 21 June 2023

    Abstract Background:Potentilla sericea is a heavy metal hyperaccumulator landscaping plant. MYB transcription factors play an important role in regulating plant stress response to adversity. However, there are few studies on MYB transcription factors in stress tolerance in Potentilla sericea. In this study, the gene was successfully cloned from Potentilla sericea. Methods: Bioinformatic analysis and real-time quantitative PCR (qPCR) methods were used to evaluate this gene. The transgenic A. thaliana were obtained by flower dipping and the gene function was identified by determining physiological indicators under cadmium stress. Results: The open reading frame of is 942 bp, which encodes 313 amino… More >

  • Open Access

    ARTICLE

    Functional Analysis of the Genotypic Differences in Response of Pea (Pisum sativum L.) to Calcareous-Induced Iron Deficiency

    Sameh Barhoumi1, Hasna Ellouzi2, Abdelmajid Krouma1,3,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 521-536, 2023, DOI:10.32604/phyton.2022.023555 - 12 October 2022

    Abstract Lime-induced iron chlorosis is a major nutritional disorder causing severe plant growth and yield reduction in the calcareous soils of Tunisia. The understanding the behavior of key metabolic functions of peas on calcareous soils, the identification of useful traits of tolerance, and the exploration of the genotypic differences in response to this constraint remain the most efficient approaches due to their coast, environmental benefits, and sustainability. For this purpose, a greenhouse experiment was conducted on three pea genotypes (Alexandra: Alex, Douce de provence: DP, and Merveille de Kelvedon: MK) cultivated on calcareous soil (Fe-deficient) and… More >

  • Open Access

    ARTICLE

    Bioinformatics and Functional Analysis of High Oleic Acid-Related Gene GmSAM22 in Soybean [Glycine max (L.) Merr.]

    Shuo Qu1, Qi Cai2, Huimin Cui1, Lamboro Abraham1, Yaolei Jiao1, Guilong Ma2, Piwu Wang1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 501-519, 2023, DOI:10.32604/phyton.2022.023479 - 12 October 2022

    Abstract High yield, high quality, stable yield, adaptability to growth period, and modern mechanization are the basic requirements for crops in the 21st century. Soybean oleic acid is a natural unsaturated fatty acid with strong antioxidant properties and stability. Known as a safe fatty acid, it has the ability to successfully prevent cardiovascular and cerebrovascular disorders. Improving the fatty acid composition of soybean seeds, can not only speed up the breeding process of high-quality high-oil and high-oleic soybeans, but also have important significance in human health, and provide the possibility for the development of soybean oil… More >

  • Open Access

    ARTICLE

    Construction and Functional Analysis of CRISPR/Cas9 Vector of FAD2 Gene Family in Soybean

    Zhifeng Xiao, Yukun Jin, Qi Zhang, Abraham Lamboro, Baozhu Dong, Zhuoyu Yang, Piwu Wang*

    Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 349-361, 2022, DOI:10.32604/phyton.2022.017451 - 26 September 2021

    Abstract Soybean oleic acid content is one of the important indexes to evaluate the quality of soybean oil. In the synthesis pathway of soybean fatty acids, the FAD2 gene family is the key gene that regulates the production of linoleic acid from soybean oleic acid. In this study, CRISPR/Cas9 gene editing technology was used to regulate FAD2 gene expression. Firstly, the CRISPR/Cas9 single knockout vectors GmFAD2-1B and GmFAD2-2C and double knockout vectors GmFAD2-2A-3 were constructed. Then, the three vectors were transferred into the recipient soybean variety Jinong 38 by Agrobacterium-mediated cotyledon node transformation, and the mutant plants were obtained. More >

  • Open Access

    ARTICLE

    Heterologous expression of bacteriocin E-760 in Chlamydomonas reinhardtii and functional analysis

    Quezada-Rivera JJ1, RE Soria-Guerra2, FS Pérez-Juárez2, L Martínez-González2, SE Valdés- Rodríguez3, NL Vasco-Méndez1, JF Morales-Domínguez1

    Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 25-35, 2019, DOI:10.32604/phyton.2019.04549

    Abstract The use of antimicrobial peptides (AMPs) synthesized by bacteria (bacteriocins) is an alternative for combating multidrug resistant bacterial strains and their production by recombinant route is a viable option for their mass production. The bacteriocin E-760 isolated from the genus Enterococcus sp. has been shown to possess inhibitory activity against Gram-negative and Gram-positive bacteria. In this study, the expression of a chimeric protein coding for E-760 in the nucleus of C. reinhardtii was evaluated, as well as, its antibacterial activity. The synthetic gene E-760S was inserted into the genome of C. reinhardtii using Agrobacterium tumefaciens.… More >

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