Open Access iconOpen Access



Cloning of and analysis of cadmium resistant in Potentilla sericea


1 College of Landscape Architecture, Northeast Forestry University, Harbin, 150040, China
2 Research Center for Economic Plant Pests, Heilongjiang Forest Protection Institute, Harbin, 150040, China

* Corresponding Author: JIANHUI WU. Email: email

(This article belongs to the Special Issue: Physiology and Molecular Biology of Plant Stress Tolerance)

BIOCELL 2023, 47(7), 1571-1582.


Background:Potentilla sericea is a heavy metal hyperaccumulator landscaping plant. MYB transcription factors play an important role in regulating plant stress response to adversity. However, there are few studies on MYB transcription factors in stress tolerance in Potentilla sericea. In this study, the gene was successfully cloned from Potentilla sericea. Methods: Bioinformatic analysis and real-time quantitative PCR (qPCR) methods were used to evaluate this gene. The transgenic A. thaliana were obtained by flower dipping and the gene function was identified by determining physiological indicators under cadmium stress. Results: The open reading frame of is 942 bp, which encodes 313 amino acids (aa) and belongs to the R2R3 MYB transcription factor. The plant overexpression vector PBI121-PsMYB62-GFP was constructed and successfully transferred into A. thaliana. The relative expression level of PsMYB62was significantly increased by CdCl2, NaCl, ABA, and mannitol treatments. The germination rate of transgenic seeds was higher than those of wild type (WT) and empty vector (EV) under different concentrations of cadmium treatment. Upon treatment with 100 μmol·L-1 of CdCl2·2.5H2O, the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in the transgenic plants were significantly higher than those in the WT and EV. The contents of H2O2, O2·- and malondialdehyde (MDA) in transgenic lines were increased, but lower than those in WT and EV. The expression levels of AtGSH, AtPCS, and AtNAS4 that were related to the regulation of cadmium were increased, but the expression levels of transgenic lines were higher than those of WT and EV. Conclusion: The above results showed that could be induced by cadmium and could improve the cadmium resistance of plants.


Cite This Article

APA Style
FENG, Z., GAO, B., GAO, Y., WU, J. (2023). Cloning of and analysis of cadmium resistant in potentilla sericea. BIOCELL, 47(7), 1571-1582.
Vancouver Style
FENG Z, GAO B, GAO Y, WU J. Cloning of and analysis of cadmium resistant in potentilla sericea. BIOCELL . 2023;47(7):1571-1582
IEEE Style
Z. FENG, B. GAO, Y. GAO, and J. WU "Cloning of and analysis of cadmium resistant in Potentilla sericea," BIOCELL , vol. 47, no. 7, pp. 1571-1582. 2023.

cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 545


  • 369


  • 0


Share Link