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    ARTICLE

    Micropropagation of Daylily (Hemerocallis fulva) from Crown-Tip Explants and Assessment of Somaclonal Variation of in Vitro-Propagated Plants Using SCoT Markers

    Esraa E. Shalan1, Said S. Soliman1, Ahmed A. Mahmoud1, Jameel M. Al-Khayri2,*, Salha M. ALshamrani3, Fatmah A. Safhi4, Areej S. Jalal4, Diaa Abd El-Moneim5, Abdallah A. Hassanin1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2183-2196, 2023, DOI:10.32604/phyton.2023.028537

    Abstract Determination of the somaclonal variation of in vitro-propagated plants is crucial to determine the appropriate micropropagation protocol and growth regulators for commercial scale multiplication. In this research, nine multiplication media (MM) augmented with different concentrations of 6-benzyl adenine (BA), Kinetin (Kin), and Thidiazuron (TDZ), Three rooting media (RM) supplemented with three levels of α-naphthalene acetic acid (NAA) and three types of soil mixtures (v/v); Coco peat/Vermiculite/Sand (CVS), Peat moss/Perlite/Sand (PPS) and Peat moss/Perlite (PP) were used in the micropropagation protocol of daylily plants. MM2 showed the maximum shoot length and the number of leaves, while MM9 showed the maximum number… More >

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