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  • Open Access


    A Simple Clear Technique in Observing Vascular Development of Grape Ovary

    Teng Fei1, Youmei Li1, Bo Li2, Zhaosen Xie1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2117-2132, 2023, DOI:10.32604/phyton.2023.028208

    Abstract The vascular system of the grapevine (Vitis vinifera L.) flower is a channel for transporting water and nutrients to the ovary. It plays an important role in the development of the ovary and fertilization through pollination. However, the vascular bundles in the flower are so tiny that they are difficult to sample and observe by traditional slicing techniques. In this study, ‘Summer Black’ grape flowers were selected as the test materials, and the tissue samples were treated by the optical clearing technique. After simple compaction, the structure and development of the vasculature were observed by common microscopy, fluorescence microscopy and… More >

  • Open Access


    Observation of Flowering Process of Grape (Vitis vinifera L.)—Insight into the Starting of Pollination of Flower Hiding in Calyptras (Cap)

    Yue Wang1,#, Xiujie Li2,#, Yusen Wu2, Zhonghui Cai1, Bo Li2, Zhaosen Xie1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1859-1871, 2023, DOI:10.32604/phyton.2023.027067

    Abstract It is generally agreed that many Vitis vinifera L. cultivars are self-fertile, where self-pollination often occurs before capfall in a process called cleistogamy. Therefore, it is difficult to identify the right time to remove stamens before self-pollination during the cross-breeding of grape. For this paper, we observed the process of grape flowering and measured the pollen viability and stigma receptivity of grape flowers of ‘Shine Muscat’ in order to identify the starting time of self-pollination before capfall and to provide useful information for improving the efficiency of cross-breeding. The results demonstrate that the anther is not cracked during the visible… More >

  • Open Access


    VvAGAMOUS Affect Development of Four Different Grape Species Ovary

    Pengfei Zhang1,#, Yuqin Zhang1,#, Qifeng Zhao2, Tiequan Niu1, Pengfei Wen1,*, Jinjun Liang1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1125-1138, 2023, DOI:10.32604/phyton.2023.026227

    Abstract Grape pistil has an important influence on fruit size and quality. However, there were few studies on grape ovary, and the development process of the ovary is still unclear. Therefore, in this paper, four different grape varieties with different lengths of small inflorescences, namely ‘Musct Hambourg’ grape (Vitis vinifera), ‘Concord’ grape (Vitis labrusca), ‘ShanPuTao’ grape (Vitis amurensis) and ‘GongNiang2Hao’ grape (Vitis amurensis × Vitis vinifera) were used as test materials. Four varieties ovary were significant differences by means of stereomicroscope, paraffin section. The expression of ovary determining gene VvAGAMOUS (VvAG) and its development related genes VvCRABS CLAW (VvCRC) and VvAGAMOUS-LIKE 11 … More >

  • Open Access


    An Approximation for the Entropy Measuring in the General Structure of SGSP3

    Kamel Jebreen1,2,3,*, Muhammad Haroon Aftab4, Mohammad Issa Sowaity5, Zeeshan Saleem Mufti4, Muhammad Hussain6

    CMC-Computers, Materials & Continua, Vol.73, No.3, pp. 4455-4463, 2022, DOI:10.32604/cmc.2022.030246

    Abstract In this article, we calculate various topological invariants such as symmetric division degree index, redefined Zagreb index, VL index, first and second exponential Zagreb index, first and second multiplicative exponential Zagreb indices, symmetric division degree entropy, redefined Zagreb entropy, VL entropy, first and second exponential Zagreb entropies, multiplicative exponential Zagreb entropy. We take the chemical compound named Proanthocyanidins, which is a very useful polyphenol in human’s diet. They are very beneficial for one’s health. These chemical compounds are extracted from grape seeds. They are tremendously anti-inflammatory. A subdivision form of this compound is presented in this article. The compound named… More >

  • Open Access


    Genome-Wide Identification and Expression Profiling of the Shaker K+ Channel and HAK/KUP/KT Transporter Gene Families in Grape (Vitis vinifera L.)

    Han Lei1,#, Junlin Li2,#, Zhizhong Song1,3,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.11, pp. 2519-2536, 2022, DOI:10.32604/phyton.2022.021268

    Abstract Potassium (K+) is an essential macronutrient for plants to maintain normal growth and development. Shaker-like K+ channels and HAK/KUP/KT transporters are critical components in the K+ acquisition and translocation. In this study, we identified 9 Shaker-like K+ channel (VvK) and 18 HAK/KUP/KT transporter (VvKUP) genes in grape, which were renamed according to their distributions in the genome and relative linear orders among the distinct chromosomes. Similar structure organizations were found within each group according to the exon/intron structure and protein motif analysis. Chromosomal distribution analysis showed that 9 VvK genes and 18 VvKUP genes were unevenly distributed on 7 or… More >

  • Open Access


    Phylogenetic analysis and target gene prediction of miR477 gene family in grape


    BIOCELL, Vol.46, No.4, pp. 941-949, 2022, DOI:10.32604/biocell.2022.016718

    Abstract To understand the molecular characteristics of the miR477 gene family of grape (Vvi-miR477) and to predict its target genes, the Vvi-miR477 genes were identified from previous small RNA sequencing data, then phylogenetic analysis and prediction of target gene were conducted. The Vvi-miR477 family consists of two precursor sequences and three mature sequences. The miR477 family members were mostly 19-22nt in length. The sequence is relatively conservative. Vvi-MIR477a and Vvi-MIR477b are located on chromosomes 1 and 2, respectively. These precursor sequences can form the typical stable stem-loop structure. Their minimum folding free energy is −39.10 kcal/mol and −50.90 kcal/mol, respectively. The… More >

  • Open Access


    Anthocyanin Profiles in Grape Berry Skins of Different Species of Wine Grapes in Shanxi, China

    Wei Tan1, Mingxiu Xu1, Siqi Xie1, Yan Zhang1, Shuai Wu1, Qinyan Zou1, Qifeng Zhao2, Qingliang Li3,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 553-570, 2021, DOI:10.32604/phyton.2021.014082

    Abstract To understand the anthocyanin characteristics of wine grape varieties, the anthocyanin composition and content of 31 wine grape varieties were analyzed to explore the use of anthocyanins as chemical fingerprints to distinguish varieties. Results showed that a total of 21 anthocyanins were detected in the skins, including cyanidin, delphinidin, petunidin, peonidin and malvidin 3-monoglucosides (or 3,5-diglucosides) along with the corresponding acetyl and p-coumaroyl derivatives. The highest and lowest total amount of anthocyanins were detected in ‘Ruby Cabernet’ and ‘Muscat Rouge’, respectively. In the 21 Vitis vinifera grapes, there were 3~11 monoglucoside anthocyanins detected, however, there were 4 to 9 monoglucoside… More >

  • Open Access


    Grapevine Phenological Quantitative Trait SSR Genotyping Using High-Throughput HRM-PCR Analysis

    Murad Awad1, Photini V. Mylona2, Alexios N. Polidoros1,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 905-923, 2020, DOI:10.32604/phyton.2020.011235

    Abstract Discrimination among grapevine varieties based on quantitative traits, such as flowering, veraison and ripening dates is crucial for variety selection in the context of climate change and in breeding programs. These traits are under complex genetic control for which 6 linked SSR loci (VVS2, VVIn16, VMC7G3, VrZAG29, VMC5G7, and VVIB23) have been identified. Using these markers in HRM-PCR analysis, we assessed genetic diversity among a large collection of 192 grapevine varieties. The grapevine germplasm used encompasses the majority of Greek vineyard with 181 varieties, 3 prominent foreign varieties and 11 varieties of Palestinian origin. The SSR markers used were highly… More >

  • Open Access


    Micropropagation of wild grapevines (Vitis spp.) of Central Mexico

    Jiménez-Martínez JH1, MG Gutiérrez-Martínez2, O Franco-Mora1, A González-Huerta2, AT Gutiérrez-Ibáñez2

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 107-112, 2013, DOI:10.32604/phyton.2013.82.107

    Abstract Two accessions of wild grapevines (Vitis spp.) native to Puebla State, Nealticán and Teziutlán, and other two native to Temascaltepec (1 and 2), Mexico State, were cultured in vitro using both Murashige-Skoog (MS) and woody plant medium (WPM) mediums. There were no differences between mediums for shoot generation. However, differences were shown among accessions for explant vigour, callus development, number of roots, leaves and nodes per explant. Rooting was evaluated only in MS medium by adding naphthalene acetic acid (NAA), indole butyric acid (IBA) or indole acetic acid (IAA) at 0.5 mg/L. With the exception of Temascaltepec 2, NAA generated… More >

  • Open Access


    Brief Note : Improved in vitro embryo development of stenospermic grape by putrescine


    BIOCELL, Vol.26, No.2, pp. 263-266, 2002, DOI:10.32604/biocell.2002.26.263

    Abstract The goal of this study was to determine the effect of putrescine, added to the culture medium, on the in vitro development of stenospermic grape (Vitis vinifera L) embryos. The cross breedings of Perlón x G.C88552 and Perlón x Argentina were used. 0 (control), 2 and 4 mM of putrescine were added to the immature seed’s culture medium. In Perlón x Argentina, 2mM of putrescine statistically increased the percentage of total embryos, direct germination, polyembryos and normal plants. In Perlón x G.C88552, only 2 mM of putrescine increased all the variables considered, eventually tripling the percentage of normal plants obtained.… More >

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