WEIYU CHEN1,2, MUHSIN H. YOUNIS3, ZHONGKUO ZHAO1,*, WEIBO CAI3,*
BIOCELL, Vol.46, No.8, pp. 1789-1801, 2022, DOI:10.32604/biocell.2022.018197
Abstract The knowledge of interactions among functional proteins helps researchers understand disease mechanisms
and design potential strategies for treatment. As a general approach, the fluorescent and affinity tags were employed
for exploring this field by labeling the Protein of Interest (POI). However, the autofluorescence and weak binding
strength significantly reduce the accuracy and specificity of these tags. Conversely, HaloTag, a novel self-labeling
enzyme (SLE) tag, could quickly form a covalent bond with its ligand, enabling fast and specific labeling of POI.
These desirable features greatly increase the accuracy and specificity, making the HaloTag a valuable system More >
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