Guan Z-J1,2,*, B Guo1,*, Y-L Huo3, J-K Dai4, Y-H Wei1
Phyton-International Journal of Experimental Botany, Vol.81, pp. 51-58, 2012, DOI:10.32604/phyton.2012.81.051
Abstract The initiation and development of organogenic buds and somatic embryos in HBsAg-transgenic cherry tomato (Lycopersicum esculentum var. cerasiforme) mutant were studied histologically. The leaf explants of the mutant were cultured on Murashige & Skoog (MS) basal medium supplemented with 6-BA 1.0 mg/L and IAA 0.05 mg/L for callus induction. Histological studies on the leaf explants of the mutant at various developmental stages revealed that organogenic buds first appeared in the axillary position of explants on the 14th cultured day, and then somatic embryos formed in the same mutant explants after 35 days of culture. Transmission electron microscopy and scanning electron… More >
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