Ting Yu*1, Lei Li†1, Wenyan Liu*, Bailiu Ya*, Hongju Cheng*, Qing Xin*
Oncology Research, Vol.27, No.5, pp. 525-532, 2019, DOI:10.3727/096504018X15179668157803
Abstract Hypoxia-induced chemoresistance is a major obstacle in the development of effective cancer therapy. In our
study, the reversal abilities of NADPH oxidase 4 (NOX4) silence on hypoxia resistance and the potential
mechanism were investigated. Our data showed that the expression of NOX4 was upregulated in human neuroblastoma cells SH-SY5Y under hypoxia condition time dependently. Knockdown of NOX4 expression by
siRNA inhibited glycolysis induced by hypoxia through decreasing the expression of glycolysis-related proteins (HIF-1 , LDHA, and PDK1), decreasing glucose uptake, lactate production, and ROS production, while
increasing mitochondria membrane potential. Moreover, NOX4 silence inhibited cell growth under hypoxia
condition through… More >
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