DONGMEI GAO1,#, JIAOE CHEN2,#, HONGZHANG LI2,*, JUN ZHAO3,*
BIOCELL, Vol.46, No.10, pp. 2275-2280, 2022, DOI:10.32604/biocell.2022.021008
- 13 June 2022
Abstract Introduction: Human cytomegalovirus (HCMV) is reported to be involved in the occurrence of many human diseases. To further investigate the biological changes of HCMV, we analyzed the relevant factors that affect the autophagy caused by HCMV infection. Methods: Firstly, we cultured human embryonic lung fibroblasts (HELF) cells with HCMV infection, and evaluated the effects of HELF cells infected with different viruses through Enzyme-linked immunoabsorbent assay (ELISA), Real-time quantitative Polymerase Chain Reaction (RT-qPCR), Acridine orange (AO) staining and Western blotting (WB) experiments. Results: Through the above experiments, we found that the combined treatment of HCMV infection and carbamazepine, More >
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