WEI ZHAI^1,2, XIANG ZHENG², MENGYUAN LIU², QIURONG ZHAO², YUSHU ZHANG², YUQING LUO³, SHANSHAN FENG³, JINJING WANG³, XUEYING LI^2,*, MINGSONG WU^1,*

BIOCELL, Vol.47, No.8, pp. 1821-1833, 2023, DOI:10.32604/biocell.2023.027175

Abstract Objective: Endoplasmic reticulum-Golgi intermediate compartment 3 (ERGIC3) promotes cell proliferation and metastasis in lung cancer, but its molecular mechanism is unclear. Methods: The GLC-82 cells were randomly divided into the ERGIC3i group and the negative control group. The cells were transfected with ERGIC3 siRNA or control siRNA in the groups, respectively. The ERGIC3-interacting proteins expressed in cells or extracellularly were isolated by the immunoprecipitation method and detected by isobaric tags for relative and absolute quantitation and liquid chromatography-tandem mass spectrometry. The differentially expressed proteins were determined by bioinformatic methods. Results: After ERGIC3 knockdown, 88 extracellular differentially expressed proteins, 41 up-regulated… More >

QIAN CHEN^1,#, XIN LEI^1,#, GUANHUA HU^1,#, YAN WANG², ZHENGQING FANG¹, GUOQUAN WANG¹, HANG SONG¹, SHU YE^1,*, BIAO CAI^1,*

BIOCELL, Vol.47, No.6, pp. 1265-1277, 2023, DOI:10.32604/biocell.2023.028811

Abstract Background: Huang-Pu-Tong-Qiao formula (HPTQ), a traditional Chinese herbal formula, has a variety of pharmacological effects. It has been used to treat Alzheimer’s disease (AD) for decades. This study aimed to screen differentially expressed proteins in the hippocampus of AD model rats treated with HPTQ. Proteomic studies of the effects of HPTQ on AD are key to understanding the therapeutic mechanisms of HPTQ and identifying potential therapeutic targets. Methods: We hence used the isobaric tags for relative and absolute quantification (ITRAQ) approach to investigate the differentially expressed proteins in the hippocampus of AD model rats before and after HPTQ administration and… More >