LINMEI ZHENG¹, RONG TANG², ^1,*, ZHONGYI ZHOU³

BIOCELL, Vol.45, No.5, pp. 1231-1240, 2021, DOI:10.32604/biocell.2021.015752

Abstract Pre-eclampsia is characterized by an excessive maternal inflammatory response. The cholinergic anti-inflammatory pathway (CAP) has been shown as the efferent arm of a vagal reflex with the potential to limit inflammatory responses. Therefore, in this study, the CAP regulation through the nervous vagal stimulation (VNS) reduced the severity of NG-nitro-L-arginine methyl ester (L-NAME)-induced pre-eclampsia was determined in a rat model. Rats were given 125 mg/kg/day of L-NAME via subcutaneous injection on gestational day (GD) 10–16. In addition, the rats were treated by active or sham electrical stimulation once a day during GD 13–19. Systolic blood pressure (SBP), urinary albumin, and… More >

JINGPENG ZHOU^1,2,#, XIAOGUANG JI^3,#, YUHAO WANG^1,2, XIAOLONG WANG^1,2, YONGJIANG MAO^1,2,*, ZHANGPING YANG^1,2,*

BIOCELL, Vol.45, No.4, pp. 1033-1044, 2021, DOI:10.32604/biocell.2021.015586

Abstract Cow mastitis is the most common disease that affects the dairy farming industry and causes serious harm to dairy cows and humans, and Staphylococcus (S.) aureus is one of the main pathogens that cause mastitis in dairy cows. In this study, a mastitis model was established through the infection of bovine mammary epithelial cells (BMECs) with S. aureus (bacterial concentration of 1 × 10⁹/mL), and these cells and a blank group (untreated) were analyzed by flow cytometry (10000 cells, 200 cells collected per second), hematoxylin and eosin (H&E) staining and immunohistochemistry. In addition, the lncRNAs (long non-coding RNAs) in the… More >

JIGANG LEI^1,#, ZHENYAO XU^2,#, SUKE LI¹, MENG LI¹, ZHIKAI WANG³, PING LI¹, JING WANG¹, YINGLU CHEN¹, XIAOLE SONG¹, CHENGJIE REN¹, MEIPING SHEN¹, CHENGXIANG DAI^1,*

BIOCELL, Vol.45, No.3, pp. 537-546, 2021, DOI:10.32604/biocell.2021.014569

Abstract Psoriasis is an autoimmune-related chronic inflammatory disease with an approximate prevalence of 2–3% around the world, involving increased keratinocyte proliferation. Indeed, Th17 cells and IL-17 play critical roles in the pathogenesis of psoriasis. The monoclonal antibodies against cytokines have been shown to have effectively immunosuppressive effects on human psoriasis. However, there are still some patients that have no response to these treatments. Some patients have even serious side-effects which may affect their life. Mesenchymal stem cells have the ability of immunosuppressive and anti-inflammatory effects, which may be an alternative therapy with more safety and efficacy for human psoriasis. Moreover, the… More >

JINGRONG SONG^#, JUAN KANG^#, WEI LV, YAN DONG, XIAOYING ZHANG^*

BIOCELL, Vol.45, No.3, pp. 723-731, 2021, DOI:10.32604/biocell.2021.014453

Abstract Bronchial asthma is a common chronic inflammatory disease characterized by airway hyperresponsiveness (AHR), inflammatory cell infiltration, and airway remodeling. F-box/WD repeat-containing protein 7 (FBXW7), an E3 ubiquitin ligase, is required for various endothelial functions, such as cell migration, inflammation, and endothelial integrity. This study aimed to investigate the role of FBXW7 in lipopolysaccharide (LPS)-induced epithelial barrier impairment in bronchial epithelial cells in vitro. By using lentivirus-based technology, FBXW7 was overexpressed or silenced (24 h) in human bronchial epithelial (16HBE) cells, which were treated with LPS or not (24 h). Immunoprecipitation (IP) detection and Western blot analysis were used to evaluate… More >

LUCIANA MAZZEI^1,2, MARÍA BELÉN RUIZ-ROSO³, NATALIA DE LAS HERAS³, SANDRA BALLESTEROS³, CAROLINA TORRESPALAZZOLO⁴, LEÓN FERDER⁵, ALEJANDRA BEATRIZ CAMARGO⁴, WALTER MANUCHA^1,2,*

BIOCELL, Vol.44, No.4, pp. 671-681, 2020, DOI:10.32604/biocell.2020.014175

Abstract The ancestral cultures have described many therapeutic properties of garlic; therefore, it is of central interest to elucidate the molecular basis explaining this millenary empirical knowledge. Indeed, it has been demonstrated a neuroprotective effect of allicin–a phytochemical present in garlic- linked to oxidative-inflammatory modulation. Allicin improved neuronal injury by heat shock protein 70 (Hsp70) and inducible nitric oxide synthase (iNOS) regulation. Also, allicin exerts renal protection involving a possible angiotensin type 1 receptor (AT1) interaction. In connection, AT1 overexpression has been recognized as a central deleterious factor in many brain diseases. However, there are no studies that evaluate AT1-Hsp70-iNOS interaction… More >

Shengwen Guo, Yuanyuan Tong, Liting Bai, Peiyao Zhang, Xin Duan^*, Jinping Liu^*

Congenital Heart Disease, Vol.15, No.5, pp. 275-285, 2020, DOI:10.32604/CHD.2020.011894

Abstract Objective: Serum ferritin has been identified as a prognostic marker in patients with a variety of diseases. In the present study we aim to determine the prevalence of risk factors and outcomes for hyperferritinemia in children undergoing cardiac surgery with cardiopulmonary bypass for congenital heart defects. Methods: The serum ferritin levels of 457 children between the ages of twentyeight days and three years undergoing cardiopulmonary bypass surgery between June 1, 2017 and June 1, 2018 were analyzed. The prevalence of early postoperative hyperferritinemia was investigated; hyperferritinemia was defined as a ferritin level ≥250 ng/ml. Multivariable regression models including candidate risk… More >

MENGSHU ZHAO¹, PINGSHI GAO¹, LIANG TAO¹, JINGJING WEN¹, LEI WANG¹, YUGUO YI¹, YUXIN CHEN², JUNSONG WANG¹, XI XU¹, JIANFA ZHANG¹, DAN WENG^1,*

BIOCELL, Vol.44, No.3, pp. 353-361, 2020, DOI:10.32604/biocell.2020.08955

Abstract As a natural analog of resveratrol, piceatannol (Pic) exhibits good antioxidant and anti-inflammatory activities in different disease models. However, the role of Pic in type 1 diabetes mouse model has not been reported yet. In this study, we investigated the in vivo effect of Pic in streptozotocin (STZ)-induced type 1 diabetic mice. Mice were injected with STZ to establish the type 1 diabetes mellitus (T1DM) model. After stable hyperglycemia was achieved, mice were then orally treated with Pic (40 mg/kg b.w., i.g.) for 30 days. The results indicated that Pic supplementation efficiently alleviated the typical symptoms associated with T1DM, including… More >

EUN NA¹, MYOUNGWON CHOI², INDAL PARK², SUNYOUNG LIM^3,*

BIOCELL, Vol.44, No.2, pp. 193-199, 2020, DOI:10.32604/biocell.2020.08648

Abstract We investigated the effect of black sea bream extracts on changes in pro-inflammatory and anti-inflammatory cytokines induced by lipopolysaccharide (LPS) in murine splenocytes. The levels of pro-inflammatory cytokines [interleukin (IL)-6, tumor necrosis factor-α (TNF-α), IL-12/IL-23(p40) and IL-17A] and anti-inflammatory cytokines [IL-4, IL-10 and interferon-γ (IFN-γ)] were assessed. Incubation of murine splenocytes with acetone+methylene chloride (A+M) and methanol (MeOH) extracts significantly decreased LPS-induced IL-6, IL-12/IL-23(p40) and IL-17A productions after 6 h incubation (p < 0.05). The A+M and MeOH extracts significantly increased LPS-induced IL-4 and IFN-γ productions at 48 and 72 h incubation (p < 0.05). Treatment with A+M extract resulted… More >

Juan Lizandro Rodríguez‐Hernández¹, Fayna Rodríguez‐González², Marta Riaño‐Ruiz³, Efrén Martínez‐Quintana^1,4

Congenital Heart Disease, Vol.13, No.5, pp. 655-662, 2018, DOI:10.1111/chd.12620

Abstract Introduction: Hyperuricemia has been associated with cardiovascular risk factors but it remains controversial if uric acid is an independent predictor of cardiac mortality.
Methods: A total of 503 CHD patients (457 nonhypoxemic and 46 hypoxemic) and 772 control patients fulfilled inclusion criteria. Demographic, clinical, and analytical data [serum uric acid and 24h urine uric acid levels, N‐terminal pro‐B‐type natriuretic peptide (NT‐pro‐BNP), and C‐reactive‐protein (CRP) concentrations] were studied. Survivals curves to determine cardiac death and arterial thrombosis in CHD patients were also examined.
Results: Noncyanotic and cyanotic CHD patients had significant higher serum uric acid concentration (5.2 ± 1.5 vs 4.9 ± 1.3mg/dL, P = .007 and… More >

Yan Gao¹, Chunli Wang¹, Li Yang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 93-93, 2019, DOI:10.32604/mcb.2019.07322

Abstract At present, anterior cruciate ligament (ACL) damage repair is still a huge challenge. Our previous studies indicated that the Lysyl oxidase (LOX) were significantly reduced in injurious ACL fibroblasts, which is the major reason for its poor healing ability. The main purpose of our study was to detected the potential of LOX to act as an anabolic agent in injured ACL. The effect of LOX on the ACL at a concentration of 20ng/mL was investigated. The molecular mechanisms and signaling pathway were elucidated by RNA-sequencing, q-PCR and western blotting. For the in vivo study, the LOX was injected into the… More >