FEI KONG¹, QIYUAN TIAN², BINGLIN KUANG³, LILI SHANG⁴, XIAOXIAO ZHANG⁵, DONGYANG LI⁵, YING KONG^6,*

BIOCELL, Vol.48, No.4, pp. 665-675, 2024, DOI:10.32604/biocell.2024.046342

Abstract Introduction: Myocardial ischemia-reperfusion (IR) injury has received widespread attention due to its damaging effects. Electroacupuncture (EA) pretreatment has preventive effects on myocardial IR injury. SLC26A4 is a Na+ independent anion reverse transporter and has not been reported in myocardial IR injury. Objectives: To find potential genes that may be regulated by EA and explore the role of this gene in myocardial IR injury. Methods: RNA sequencing and bioinformatics analysis were performed to obtain the differentially expressed genes in the myocardial tissue of IR rats with EA pretreatment. Myocardial infarction size was detected by TTC staining. Serum CK, creatinine kinase-myocardial band,… More > Graphic Abstract

RAN PAN^1,#, JIAYAN MAO^2,#, YUELIANG ZHENG³, WEI CHEN², JUNPING GUO^1,*, LIJUN WANG^3,*

BIOCELL, Vol.48, No.3, pp. 431-441, 2024, DOI:10.32604/biocell.2023.046484

Abstract Background: Sepsis, a type of systemic disease, can impact nearly all organs, tissues and cells. Among them, endothelial cells are amongst the first to be affected and respond to the insult. In this study, we investigated the protective effects of microRNA-30a-5p (miR-30a-5p) on human pulmonary microvascular endothelial cells (HPMECs) treated with lipolysaccharide (LPS). Methods: An in vitro model of sepsis was established in HPMECs with the use of LPS. Transfecting with different tools (mimetic and inhibitor) to modify miR-30a-5p expression. Cell viability, proliferation and apoptosis were detected by the CCK-8 assay, the EdU kit and fluorescence staining, respectively. The autophagy-related… More >

Saba Awan^1,*, Zahid Mehmood^2,*

CMC-Computers, Materials & Continua, Vol.78, No.2, pp. 2535-2555, 2024, DOI:10.32604/cmc.2024.047337

Abstract Highway safety researchers focus on crash injury severity, utilizing deep learning—specifically, deep neural networks (DNN), deep convolutional neural networks (D-CNN), and deep recurrent neural networks (D-RNN)—as the preferred method for modeling accident severity. Deep learning’s strength lies in handling intricate relationships within extensive datasets, making it popular for accident severity level (ASL) prediction and classification. Despite prior success, there is a need for an efficient system recognizing ASL in diverse road conditions. To address this, we present an innovative Accident Severity Level Prediction Deep Learning (ASLP-DL) framework, incorporating DNN, D-CNN, and D-RNN models fine-tuned through iterative hyperparameter selection with Stochastic… More >

QINGJIAN HE¹, HUIXIN ZHU^2,3, SHANHONG FANG^4,5,*

BIOCELL, Vol.48, No.2, pp. 205-216, 2024, DOI:10.32604/biocell.2023.045109

Abstract Introduction: Dexamethasone (Dex) caused impaired osteoblast differentiation and oxidative stress (OS) in bone marrow mesenchymal stem cells (BMSCs). This work sought to elucidate the precise molecular pathway through which Dex influences osteogenic differentiation (OD) and OS in BMSCs. Methods: The expression of Runt-related transcription factor 1 (RUNX1) and alpha-2 macroglobulin (A2M) was assessed in Dex-treated BMSCs using qRT-PCR and Western Blot. Following the functional rescue experiments, cell proliferation was determined by MTT assay, reactive oxygen species (ROS) expression by DCFH-DA fluorescent probe, lactate dehydrogenase (LDH), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (Gpx) expression by kits, OD by alkaline… More >

WEI ZHENG¹, CHENG LIU^2,*

BIOCELL, Vol.48, No.2, pp. 293-301, 2024, DOI:10.32604/biocell.2023.044211

Abstract Objective: Intracranial aneurysm (IA) represents a devastating disease with high rates of disability and mortality, which is initiated by dysfunction of endothelial cells (ECs). Evidence suggests the dysregulation of the E3 ubiquitin ligase family during EC injury. In this work, the role of an E3 ubiquitin ligase, casitas B lymphoma-B (CBL-B), was explored in human brain microvascular EC (HBMEC) function through the NLRP3 pathway. Methods: In vitro IA model was induced by treating HBMECs with oxidized low-density lipoprotein (ox-LDL). The levels of CBL-B and pyroptosis-related proteins NLRP3, ASC, cleaved caspase-1, and GSDME-N were determined by real-time-quantitative polymerase chain reaction and… More >

Yuhan Zhou^1,2,#, Xiaojun Li^3,#, Yanhui Xiang^1,2,4,*

International Journal of Mental Health Promotion, Vol.26, No.1, pp. 31-38, 2024, DOI:10.32604/ijmhp.2023.043167

Abstract Fathers play an important role in children’s development throughout their lives, and Father-Love Absence (FLA) leads to more behavioral problems, including Non-Suicidal Self-Injury (NSSI). However, there has been no research on the relationship between FLA and NSSI and its influencing mechanism. This study is based on Nock’s integrated theoretical model of NSSI, aiming to explore the influence of FLA on NSSI and further investigate the mechanism of Subjective Vitality (SV) and Forgiveness (FORG). This study recruited 1795 adolescents in central China to complete four scales, including Father-Love Absence Scale (FLAS), The Subjective Vitality Scale (SVS), The Tendency to Forgive Scale… More >

PING YANG^1,2,3, YUANXIANG LAN^1,2, ZHONG ZENG^1,2, YAN WANG^1,2, HECHUN XIA^1,2,*

BIOCELL, Vol.48, No.1, pp. 149-162, 2024, DOI:10.32604/biocell.2023.042367

Abstract Background: As a form of biological therapy, placenta-derived mesenchymal stem cells (PDMSCs) exhibit considerable promise in addressing the complex pathological processes of traumaticbrain injury (TBI) due to their multi-target and multi-pathway mode of action. Material & Methods: This study investigates the protective mechanisms and benefits of PDMSCs in mitigating the effects of controlled cortical impact (CCI) in rats and glutamate-induced oxidative stress injury in HT22 cells in vitro. Our primary objective is to provide evidence supporting the clinical application of PDMSCs. Results: In the in vivo arm of our investigation, we observed a swift elevation of matrix metalloproteinase-9 (MMP-9) in… More > Graphic Abstract

YANG GU^1,#,*, MINGGUANG BI^2,#, DEHUI CHEN³, NING NI⁴, JIANMING CHEN^1,*

BIOCELL, Vol.47, No.9, pp. 2069-2080, 2023, DOI:10.32604/biocell.2023.029989

Abstract Background: The underlying mechanism of sciatic nerve injury (SNI) is a common motor functional disorder, necessitates further research. Methods: A rat model of SNI was established, with the injury group subjected to compressive injury of the right sciatic nerve exposed at the midpoint of the thigh and the sham surgery group undergoing the same surgical procedure. An oxygen-glucose deprivation model was employed to simulate in vitro SNI in PC12 cells. Following data acquisition and quality control, differentially expressed proteins (DEPs) in each model were identified through differential analysis, and enrichment analysis was used to explore the potential functions and pathways… More >

CHUNYANG XU^1,#, CAIYUN YANG^1,#, JINSONG ZHANG², XIAOHUA PAN³, JUN WANG⁴, LEI JIANG², HONGWEI YE^1,*, BO CHEN^1,*

BIOCELL, Vol.47, No.9, pp. 2027-2035, 2023, DOI:10.32604/biocell.2023.029277

Abstract Background: The lung is one of the primary target organs of hydrogen sulfide (H₂S), as exposure to H₂S can cause acute lung injury (ALI) and pulmonary edema. Dexamethasone (Dex) exerts a protective effect on ALI caused by exposure to toxic gases and is commonly used in the clinic; however, the underlying mechanisms remain elusive, and the dose is unclear. Methods: In vivo experiments: divided C57BL6 mice into 6 groups at random, 12 in each group. The mice were exposed to H₂S for 3 h and 5 or 50 mg/kg Dex pretreated before exposure, sacrificed 12 h later. The morphological changes… More >

Ruoyi Ni^#, Hao Dou^#, Shuwen Bi, Tiantian Wang, Jin’e Quan^*, Xitian Yang^*

Phyton-International Journal of Experimental Botany, Vol.92, No.10, pp. 2907-2920, 2023, DOI:10.32604/phyton.2023.030689

Abstract The objective of this study was to investigate the effects of root cutting stress on the dynamic changes of endogenous hormone content and growth characteristics of Quercus variabilis roots, and to explore the physiological role of endogenous hormones in regulating root-crown interactions in the short term. The morphological characteristics and endogenous hormone contents of normal roots (no root cutting, CK) and cut roots (cut by 1/3 of the length of the main root, RP) were determined by liquid chromatography, which was combined with mass spectrometry at different levels of different developmental stages. The results showed that the root growth indexes… More >