ZHIHENG LIU^1,2, ZHEN SUN³, ZHONGYUAN WAN⁴, HAIQIANG WANG^2,*

BIOCELL, Vol.46, No.2, pp. 511-517, 2022, DOI:10.32604/biocell.2021.016194

Abstract We unraveled the expression profiles of coding-noncoding RNAs in intervertebral disc degeneration (IDD). However, it remains elusive regarding miR-155 expression in peripheral blood mononuclear cells (PBMCs) and local extracellular space in IDD. The study aimed for investigating the miR-155 expression of PBMCs, extracellular miRNAs (ex-miRNAs) of human nucleus pulposus (NP) tissues, and morphological changes of cell death in the IDD process. Here, we harvested peripheral blood and NP samples from scoliosis and IDD patients as control and degenerative groups, respectively. Then standard Ficoll density-gradient centrifugation was used to isolate PBMCs. The two subpopulations of PBMCs were divided based on the… More >

Runze Zhao¹, Tingting Xia¹, Mengyue Wang¹, Fan Feng¹, Wanqian Liu^1,*, Li Yang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 77-77, 2019, DOI:10.32604/mcb.2019.07155

Abstract This study was focus on the exploring the therapeutic function of lysyl oxidase (LOX) in rat nucleus pulposus (NP) cells in intervertebral disc degeneration (IVDD). To do this, a Sprague-Dawley (SD) rat caudal spine degeneration model was established by puncturing the Co5-6 disc. NP cells apoptosis and extracellular matrix (ECM) degeneration in IVDD were evaluated by real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR), Hematoxylin-Eosin (H&E) and immunofluorescence. Then, the therapeutic effect of LOX on IVDD was evaluated by histological staining. In vitro, the regulator effect of LOX on degenerate rat NP cell was explored. ECM relate proteins and cytokines were… More >