SHUHUI WU1,2,#, PING XU3,#, XIANGBIN XU2, SONG-BAI LIU1,2,*
BIOCELL, Vol.47, No.11, pp. 2385-2395, 2023, DOI:10.32604/biocell.2023.029687
Abstract Nucleic acid (DNA and RNA) detection and quantification methods play vital roles in molecular biology. With
the development of molecular biology, isothermal amplification of DNA/RNA, as a new molecular biology technology,
can be amplified under isothermal condition, it has the advantages of high sensitivity, high specificity, and high
efficiency, and has been applied in various fields of biotechnology, including disease diagnosis, pathogen detection,
food hygiene and safety detection and so on. This paper introduces the progress of isothermal amplification
technology, including rolling circle amplification (RCA), nucleic acid sequence-dependent amplification (NASBA),
strand displacement amplification (SDA), loop-mediated isothermal amplification (LAMP), helicase-dependent
amplification… More >
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