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  • Open Access


    Label-free quantitative proteomics analysis models in vivo and in vitro reveal key proteins and potential roles in sciatic nerve injury


    BIOCELL, Vol.47, No.9, pp. 2069-2080, 2023, DOI:10.32604/biocell.2023.029989

    Abstract Background: The underlying mechanism of sciatic nerve injury (SNI) is a common motor functional disorder, necessitates further research. Methods: A rat model of SNI was established, with the injury group subjected to compressive injury of the right sciatic nerve exposed at the midpoint of the thigh and the sham surgery group undergoing the same surgical procedure. An oxygen-glucose deprivation model was employed to simulate in vitro SNI in PC12 cells. Following data acquisition and quality control, differentially expressed proteins (DEPs) in each model were identified through differential analysis, and enrichment analysis was used to explore the… More >

  • Open Access


    Proteomic Analysis of Chrysanthemum Lateral Buds after Removing Apical Dominance Based on Label-Free Technology

    Sicong Zheng#, Jingjing Song#, Cheng Luo, Xin Li, Qiqi Ma, Beibei Jiang*, Qinglin Liu, Yuanzhi Pan

    Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 525-539, 2022, DOI:10.32604/phyton.2022.017629

    Abstract Studying the genetic basis and regulatory mechanism of chrysanthemum lateral bud outgrowth is of great significance for reduction the production cost of cut chrysanthemum. To clarify the molecular basis of lateral bud elongation after removal of apical dominance in chrysanthemum, label-free quantification analysis was used to analyze the proteome changes after apical bud removal. Quantitative real-time PCR (qPCR) was used to analyze the changes in the expression of three plant hormone-related genes. A total of 440 differentially expressed proteins were successfully identified at three time points during the lateral bud elongation. The number of differentially… More >

  • Open Access


    Real-Time analysis of exosome secretion of single cells with single molecule imaging


    BIOCELL, Vol.45, No.6, pp. 1449-1451, 2021, DOI:10.32604/biocell.2021.017607

    Abstract The exosome-mediated response can promote or restrain the diseases by regulating the intracellular pathways, making the exosome become an effective marker for diagnosis and therapeutic control at the single-cell level. However, real-time analysis is hard to be achieved with traditional approaches because the exosomes usually need to be enriched by ultracentrifugation for a measurable signal-to-noise ratio. Recently developed label-free single-molecule imaging approaches may become an real-time quantitative tool for the analysis of single exosomes and related secretion behaviors of single living cells owing to their extreme sensitivity. More >

  • Open Access


    The Dependence of Diffusio-Phoretic Mobility and Aggregation Properties of Proteins on Intermolecular Interaction in Confined System

    Jiachen Wei1,2,*

    Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 103-104, 2019, DOI:10.32604/mcb.2019.07721

    Abstract Phoretic flow can be generated by different types of gradient (e.g. temperature, concentration, or charge gradient) [1-3]. Within micro-to-nano confined system, the diffusio-phoretic property for proteins differs dramatically from that obtained in bulk condition, due to concentration fluctuation that emerges at microscopic level induced by specific and nonspecific interactions between protein and co-solute [4-5]. The phoretic mobility of protein individuals and complex in solute gradients can be theoretically described by continuum model [1-2] that neglects microscopic heterogeneity and determined experimentally by microfluidics [6], but the underlying mechanism of diffusio-phoretic motion for confined protein still remains… More >

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