Shoukai Lin^1,2,#, Shichang Xu^1,#, Liyan Huang¹, Fuxiang Qiu¹, Yihong Zheng¹, Qionghao Liu¹, Shiwei Ma^1,2, Bisha Wu^1,2, Jincheng Wu^1,2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1185-1201, 2023, DOI:10.32604/phyton.2023.026752

Abstract Loquat (Eriobotrya japonica Lindl.) is a subtropical evergreen fruit tree that produces fruits with abundant nutrients and medicinal components. Confirming suitable reference genes for a set of loquat samples before qRT-PCR experiments is essential for the accurate quantification of gene expression. In this study, eight candidate reference genes were selected from our previously published RNA-seq data, and primers for each candidate reference gene were designed and evaluated. The Cq values of the candidate reference genes were calculated by RT-qPCR in 31 different loquat samples, including 12 subgroups of developing or abiotic-stressed tissues. Different combinations of stable reference genes were screened… More >

Thiresia-Teresa Tzatzani¹, Evangelia Basdeki², Evangelia-Vasiliki Ladikou³, Marios-Ioannis N. Sotiras³, Georgios Panagiotakis⁴, Sryridon Lionakis⁴, Ioannis E. Papadakis^3,*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 645-656, 2020, DOI:10.32604/phyton.2020.010532

Abstract The purpose of this study was to investigate the effects of various presowing treatments on the germinability (final germination percentage) and germination rate of loquat seeds in order to increase seedling production in nurseries (applied research) as well as provide answers for important physiological issues related to loquat seeds and their seed coat (basic research). Three experiments were carried out with various pre-sowing treatments. These treatments included full or partial removal of seed coat (perisperm), partial cutting of cotyledons as well as moist chilling at 5°C for 13 days and/or soaking the seeds in water or 250 ppm gibberellic acid… More >