Home / Advanced Search

  • Title/Keywords

  • Author/Affliations

  • Journal

  • Article Type

  • Start Year

  • End Year

Update SearchingClear
  • Articles
  • Online
Search Results (18)
  • Open Access

    ARTICLE

    Light-controlled phosphorylation in the TrkA-Y785 site by photosensitive UAAs activates the MAPK/ERK signaling pathway

    SHU ZHAO1,*, SHIXIN YE2

    BIOCELL, Vol.47, No.6, pp. 1377-1388, 2023, DOI:10.32604/biocell.2023.023874

    Abstract Background: This paper aims to establish a light-controlled phosphorylation detection method at the Y785 site of tropomyosin receptor kinase A (TrkA) receptor in mammalian cells by using genetic code expansion technology and detecting the effects of optical activation of this site on the downstream MAPK/ERK pathway. The study is based on the current situation that the regulatory mechanism of TrkA phosphorylation has not been fully elucidated. Methods: Two photosensitive unnatural amino acids, p-azido-L-phenylalanine (AzF) and photo-caged tyrosine (ONB) were introduced into the TrkA-Y785 site by genetic code expansion technology and site-directed mutagenesis. Western blotting and laser confocal imaging were conducted… More >

  • Open Access

    ARTICLE

    Transcriptome Analysis of Auxin Drives Cone Size Regulation in Fokienia hodginsii

    Shunde Su1,2,#, Huan Chang3,#, Xiangyang Kang1,*, Renhua Zheng2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1713-1723, 2023, DOI:10.32604/phyton.2023.027586

    Abstract As the reproductive organ of the endangered species Fokienia hodginsii, the size of the cones is a constraint on the reproductive renewal of the population. In this study, the molecular basis of the influence of cone size on F. hodginsii was elucidated by comparing the phenotype, biochemistry, and transcriptome of two cultivars of F. hodginsii (‘FJ431’ and ‘FJ415’). The two cultivars differed significantly in cone size, with FJ431 having a significantly larger cone size and weight than FJ415, 1.32 and 1.90 times that of FJ415, respectively. RNA-Seq analysis of both cultivars retrieved 75,940 genes whose approximate functions were classified as… More >

  • Open Access

    ARTICLE

    Network pharmacology and molecular docking identify mechanisms of medicinal plant-derived 1,2,3,4,6-penta-O-galloyl-beta-D-glucose treating gastric cancer

    MAN REN1,2,3,#, YUAN YANG1,2,#, DAN LI4, NANNAN ZHAO5, YUPING WANG2,6,*, YONGNING ZHOU2,6,*

    BIOCELL, Vol.47, No.5, pp. 977-989, 2023, DOI:10.32604/biocell.2023.028402

    Abstract Background: 1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG) is a natural polyphenolic compound derived from multiple medicinal plants with favorable anticancer activity. Methods: In this study, the mechanisms of PGG against gastric cancer were explored through network pharmacology and molecular docking. First, the targets of PGG were searched in the Herbal Ingredients’ Targets (HIT), Similarity Ensemble Approach (SEA), and Super-PRED databases. The potential targets related to gastric cancer were predicted from the Human Gene Database (GeneCards) and DisGeNET databases. The intersecting targets of PGG and gastric cancer were obtained by Venn diagram and then subjected to protein-protein interaction analysis to screen hub targets. Functional and… More >

  • Open Access

    REVIEW

    Research progress of protein phosphatase 2A in cellular autophagy

    HONGMEI WU#, DI LI#, YUANYUAN HUANG, RUYUAN LIU, XIAONIAN ZHU*

    BIOCELL, Vol.47, No.3, pp. 485-491, 2023, DOI:10.32604/biocell.2023.026049

    Abstract Autophagy is an important metabolic process. It facilitates the recycling of intracellular substances by removing, degrading, and recycling damaged organelles, proteins, and lipids in lysosomal vacuoles and plays an important role in maintaining cellular homeostasis. Protein phosphatase 2A (PP2A) is a key serine/threonine phosphatase and one of the main cell cycle regulatory enzymes. As PP2A activity is essential for the cell, dysfunction or dysregulation of PP2A can affect various physiological processes, including autophagy. Here, we review the autophagy-related factors that target PP2A in different diseases, such as breast cancer, colorectal cancer, liver cancer, and Alzheimer’s disease, to maintain cell homeostasis… More >

  • Open Access

    ARTICLE

    Schisandrin B exerts anticancer effects on human gastric cancer cells through ROS-mediated MAPK, STAT3, and NF-κB pathways

    TIANZHU LI1,#, YU ZHANG2,#, TONG ZHANG2,#, YANNAN LI2, HUI XUE2, JINGLONG CAO2, WENSHUANG HOU2, YINGHUA LUO3,*, CHENGHAO JIN2,4,*,

    BIOCELL, Vol.47, No.1, pp. 195-204, 2023, DOI:10.32604/biocell.2023.025593

    Abstract Schisandrin B (Sch B) is a monomer with anti-cancer and anti-inflammatory effects, which are isolated from the plant Schisandra chinensis (Turcz) Baillon. We investigated the anti-gastric cancer (GC) effects of Sch B and its underlying molecular mechanisms. The Cell Counting Kit-8 assay was used to determine the effects of Sch B on the viability of GC and normal cell lines. Hoechst/propidium iodide staining and flow cytometry were used to assess the apoptosis induction of Sch B. Western blotting was used to evaluate the effects of Sch B on downstream apoptotic proteins. The DCFH-DA fluorescent probe was used to assess the… More >

  • Open Access

    ARTICLE

    Characterization and Pathogenicity of Pseudopestalotiopsis vietnamensis Causing Gray Blight of Wuyi Rock Tea (Camellia sinensis) in China and Specific Mechanisms of Disease Infection

    Guangheng Wu1,#,*, Lu Rui2,3,#, Xiang Lu4, Libo Han2, Gan Lv1, Xianyu Fu5, Jinxian Liu5, Nong Zhou3, Chuanhai Zhang1

    Phyton-International Journal of Experimental Botany, Vol.92, No.1, pp. 131-147, 2023, DOI:10.32604/phyton.2022.021919

    Abstract Gray blight disease (GBD) causes significant losses in tea production in China. Although genes and biological processes involved in resistance to fungal disease in tea plants have been identified, specific mechanisms of the GBD infection process remain unknown. In this study, morphological and multi-gene (TEF-TUB-ITS) phylogenetic characteristics were used to identify isolate CLBB1 of Pseudopestalotiopsis vietnamensis. Pathogenicity tests confirmed that isolate CLBB1 from tea leaves caused GBD in the susceptible tea cultivar Wuyi Rock (Camellia sinensis var. sinensis cv. Shuixian). Spores began to germinate 24 h after infection (hai), and after 48 h, elongated fungal hyphae formed from a single conidium. Transcriptome… More >

  • Open Access

    ARTICLE

    Metformin alleviates LTA-induced inflammatory response through PPARγ/MAPK/NF-κB signaling pathway in bovine mammary epithelial cells

    ABDELAZIZ ADAM IDRISS ARBAB1,3,#, CHUNQING YIN4,#, XUBIN LU1, YAN LIANG1, ISMAIL MOHAMED ABDALLA1, AMER ADAM IDRIS3, TIANLE XU1,2, YONGJIANG MAO1, ZHANGPING YANG1,2,*

    BIOCELL, Vol.46, No.11, pp. 2443-2454, 2022, DOI:10.32604/biocell.2022.020865

    Abstract Mastitis is a common inflammatory cow mammary infection; that causes significant economic loss in dairy industry. Given the interesting connection between metformin’s anti-inflammatory function and mastitis model induced by LTA in pbMECs, our objective was to prove that metformin was beneficial in suppressing proinflammatory response induced by LTA through modulation of mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) signaling pathways and activation of peroxisome proliferator-activated receptor-γ (PPARγ) in pbMECs. The proliferation of cells and mRNA expression were measured using EdU assay and quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Immunoblotting and immunofluorescence analysis were conducted to evaluate the… More >

  • Open Access

    ERRATUM

    Procaine Inhibits the Proliferation and Migration of Colon Cancer Cells Through Inactivation of the ERK/MAPK/FAK Pathways by Regulation of RhoA

    Chang Li*, Shuohui Gao*, Xiaoping Li, Chang Li, Lianjun Ma§

    Oncology Research, Vol.28, No.6, pp. 675-679, 2020, DOI:10.3727/096504021X16137463165406

    Abstract Colon cancer is one of the most lethal varieties of cancer. Chemotherapy remains as one of the principal treatment approaches for colon cancer. The anticancer activity of procaine (PCA), which is a local anesthetic drug, has been explored in different studies. In our study, we aimed to explore the anticancer effect of PCA on colon cancer and its underlying mechanism. The results showed that PCA significantly inhibited cell viability, increased the percentage of apoptotic cells, and decreased the expression level of RhoA in HCT116 cells in a dose-dependent manner (p < 0.05 or p < 0.01). Moreover, PCA increased the… More >

  • Open Access

    ARTICLE

    (−)-Epigallocatechin-3-Gallate Inhibits EBV Lytic Replication via Targeting LMP1-Mediated MAPK Signal Axes

    Hongde Li*†‡, Yueshuo Li*†‡, Jianmin Hu*†‡, Sufang Liu§, Xiangjian Luo*†‡¶, Min Tang*†‡, Ann M. Bode#, Zigang Dong#**, Xinqi Liu††, Weihua Liao‡‡, Ya Cao*†‡¶ §§¶¶

    Oncology Research, Vol.28, No.7-8, pp. 763-778, 2020, DOI:10.3727/096504021X16135618512563

    Abstract Epstein–Barr virus (EBV)-encoded latent membrane protein 1 (LMP1) plays an important oncogenic role in the viral latent infection. Recently, increasing evidence indicates that the high expression of LMP1 during EBV lytic cycle is related to the viral lytic replication. However, the mechanism by which LMP1 regulates EBV lytic replication remains unclear. (−)-Epigallocatechin-3-gallate (EGCG) prevents carcinogenesis by directly targeting numerous membrane proteins and effectively inhibits EBV lytic cascade. Here, we demonstrated that LMP1 promotes EBV lytic replication through the downstream signal molecules MAPKs, including ERKs, p38, and JNKs. LMP1 induces the phosphorylation of p53 through MAPKs to enhance the ability of… More >

  • Open Access

    ARTICLE

    Therapeutic Targeting PLK1 by ON-01910.Na Is Effective in Local Treatment of Retinoblastoma

    Huan Ma1, Cong Nie1, Ying Chen, Jinmiao Li, Yanjie Xie, Zhixin Tang, Yang Gao, Siming Ai, Yuxiang Mao, Qian Sun, Rong Lu

    Oncology Research, Vol.28, No.7-8, pp. 745-761, 2020, DOI:10.3727/096504021X16130322409507

    Abstract Cell cycle deregulation is involved in the pathogenesis of many cancers and is often associated with protein kinase aberrations, including the polo-like kinase 1 (PLK1). We used retinoblastoma, an intraocular malignancy that lacks targeted therapy, as a disease model and set out to reveal targetability of PLK1 with a small molecular inhibitor ON-01910.Na. First, transcriptomic analysis on patient retinoblastoma tissues suggested that cell cycle progression was deregulated and confirmed that PLK1 pathway was upregulated. Next, antitumor activity of ON-01910.Na was investigated in both cellular and animal levels. Cytotoxicity induced by ON-01910.Na was tumor specific and dose dependent in retinoblastoma cells,… More >

Displaying 1-10 on page 1 of 18. Per Page