Guangheng Wu^1,#,*, Lu Rui^2,3,#, Xiang Lu⁴, Libo Han², Gan Lv¹, Xianyu Fu⁵, Jinxian Liu⁵, Nong Zhou³, Chuanhai Zhang¹

Phyton-International Journal of Experimental Botany, Vol.92, No.1, pp. 131-147, 2023, DOI:10.32604/phyton.2022.021919

Abstract Gray blight disease (GBD) causes significant losses in tea production in China. Although genes and biological processes involved in resistance to fungal disease in tea plants have been identified, specific mechanisms of the GBD infection process remain unknown. In this study, morphological and multi-gene (TEF-TUB-ITS) phylogenetic characteristics were used to identify isolate CLBB1 of Pseudopestalotiopsis vietnamensis. Pathogenicity tests confirmed that isolate CLBB1 from tea leaves caused GBD in the susceptible tea cultivar Wuyi Rock (Camellia sinensis var. sinensis cv. Shuixian). Spores began to germinate 24 h after infection (hai), and after 48 h, elongated fungal hyphae formed from a single conidium. Transcriptome… More >

ABDELAZIZ ADAM IDRISS ARBAB^1,3,#, CHUNQING YIN^4,#, XUBIN LU¹, YAN LIANG¹, ISMAIL MOHAMED ABDALLA¹, AMER ADAM IDRIS³, TIANLE XU^1,2, YONGJIANG MAO¹, ZHANGPING YANG^1,2,*

BIOCELL, Vol.46, No.11, pp. 2443-2454, 2022, DOI:10.32604/biocell.2022.020865

Abstract Mastitis is a common inflammatory cow mammary infection; that causes significant economic loss in dairy industry. Given the interesting connection between metformin’s anti-inflammatory function and mastitis model induced by LTA in pbMECs, our objective was to prove that metformin was beneficial in suppressing proinflammatory response induced by LTA through modulation of mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) signaling pathways and activation of peroxisome proliferator-activated receptor-γ (PPARγ) in pbMECs. The proliferation of cells and mRNA expression were measured using EdU assay and quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Immunoblotting and immunofluorescence analysis were conducted to evaluate the… More >

Chang Li^*, Shuohui Gao^*, Xiaoping Li^†, Chang Li^‡, Lianjun Ma^§

Oncology Research, Vol.28, No.6, pp. 675-679, 2020, DOI:10.3727/096504021X16137463165406

Abstract Colon cancer is one of the most lethal varieties of cancer. Chemotherapy remains as one of the principal treatment approaches for colon cancer. The anticancer activity of procaine (PCA), which is a local anesthetic drug, has been explored in different studies. In our study, we aimed to explore the anticancer effect of PCA on colon cancer and its underlying mechanism. The results showed that PCA significantly inhibited cell viability, increased the percentage of apoptotic cells, and decreased the expression level of RhoA in HCT116 cells in a dose-dependent manner (p < 0.05 or p < 0.01). Moreover, PCA increased the… More >

Hongde Li^*†‡, Yueshuo Li^*†‡, Jianmin Hu^*†‡, Sufang Liu^§, Xiangjian Luo^*†‡¶, Min Tang^*†‡, Ann M. Bode^#, Zigang Dong^#**, Xinqi Liu^††, Weihua Liao^‡‡, Ya Cao^{*†‡¶ §§¶¶}

Oncology Research, Vol.28, No.7-8, pp. 763-778, 2020, DOI:10.3727/096504021X16135618512563

Abstract Epstein–Barr virus (EBV)-encoded latent membrane protein 1 (LMP1) plays an important oncogenic role in the viral latent infection. Recently, increasing evidence indicates that the high expression of LMP1 during EBV lytic cycle is related to the viral lytic replication. However, the mechanism by which LMP1 regulates EBV lytic replication remains unclear. (−)-Epigallocatechin-3-gallate (EGCG) prevents carcinogenesis by directly targeting numerous membrane proteins and effectively inhibits EBV lytic cascade. Here, we demonstrated that LMP1 promotes EBV lytic replication through the downstream signal molecules MAPKs, including ERKs, p38, and JNKs. LMP1 induces the phosphorylation of p53 through MAPKs to enhance the ability of… More >

Huan Ma¹, Cong Nie¹, Ying Chen, Jinmiao Li, Yanjie Xie, Zhixin Tang, Yang Gao, Siming Ai, Yuxiang Mao, Qian Sun, Rong Lu

Oncology Research, Vol.28, No.7-8, pp. 745-761, 2020, DOI:10.3727/096504021X16130322409507

Abstract Cell cycle deregulation is involved in the pathogenesis of many cancers and is often associated with protein kinase aberrations, including the polo-like kinase 1 (PLK1). We used retinoblastoma, an intraocular malignancy that lacks targeted therapy, as a disease model and set out to reveal targetability of PLK1 with a small molecular inhibitor ON-01910.Na. First, transcriptomic analysis on patient retinoblastoma tissues suggested that cell cycle progression was deregulated and confirmed that PLK1 pathway was upregulated. Next, antitumor activity of ON-01910.Na was investigated in both cellular and animal levels. Cytotoxicity induced by ON-01910.Na was tumor specific and dose dependent in retinoblastoma cells,… More >

YUANNA DU^1,#, WENWEN GONG^2,#, JING LIANG^1,#, RUKUN ZANG¹, JUNJUN MOU¹

BIOCELL, Vol.46, No.5, pp. 1181-1188, 2022, DOI:10.32604/biocell.2022.016148

Abstract Endometrial cancer remains to be a major type of malignancy in threatening female life. Molecular insights in advancing our understanding of endometrial tumorigenesis are much needed. We here report that a less-studied protein Dihydropyrimidinase like 3 (DPYSL3) is a potent tumor suppressor. DPYSL3 is uniquely regulated by wild type p53 (wtp53), and its expression is at the highest level when cells carry wtp53 and are exposed to hypoxia. We reveal that wtp53 can bind DPYSL3 promoter to enhance DPYSL3 expression and in turn, the elevated DPYSL3 can restrain cancer cell proliferation and invasion in vitro and in vivo. Importantly, we… More >

Wuzhong Yin^#, Hongxia Yang^#, Yantong Wang, Ping Feng, Yao Deng, Yang Liu, Danyang Chen, Yijie Ban, Weichi Liu, Guanghua He^*, Nan Wang^*

Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 727-744, 2022, DOI:10.32604/phyton.2022.017811

Abstract The mitogen-activated protein kinase (MAPK) cascade is important in stress signal transduction and plant development. In the present study, we identified a rice (Oryza sativa L.) mutant with reduced fertility, Oryza sativa mitogen-activated protein kinase 6 (osmapk6), which harbored a mutated MAPK gene. Scanning and transmission electron microscopy, quantitative RT-PCR analysis, TUNEL assays, RNA in situ hybridization, longitudinal and transverse histological sectioning, and map-based cloning were performed to characterize the osmapk6 mutant. The gene OsMAPK6 was expressed throughout the plant but predominantly in the microspore mother cells, tapetal cells, and microspores in the anther sac. Compared with the wild type,… More >

PATRIC HAMBLETON¹, MANUEL ALEJANDRO BARBIERI^1,2,3,4,*

BIOCELL, Vol.44, No.4, pp. 525-534, 2020, DOI:10.32604/biocell.2020.011407

Abstract Cancerous cells display abnormalities in the signal transduction pathways responsible for responding to extracellular growth factors, or mitogens. Mutations that alter proteins involved in these types of pathways can lead to inappropriate or unregulated cell growth, and therefore predispose the cell to become malignant. The critical role of the Ras/mitogen-activated protein kinase (MAPK) pathway in transducing growth signals to the interior of the cell and subsequently stimulating cell growth and proliferation is underscored by the fact that roughly one quarter of all human tumors contain mutant forms of Ras proteins. A particular focus on the signaling and membrane trafficking adaptor… More >

WEI LIU^1,#, ZI WANG^1,2,#, JING LENG¹, HENG WEI¹, SHEN REN^1,2, XIAOJIE GONG³, CHEN CHEN⁴, YINGPING WANG^1,2, RUI ZHANG^1,2,*, WEI LI^1,2,*

BIOCELL, Vol.44, No.4, pp. 655-669, 2020, DOI:10.32604/biocell.2020.013202

Abstract Heat stress (HS) reaction can lead to serious physiological dysfunction associated with cardiovascular and various organ diseases. Ginsenoside Rg3 (G-Rg3) is a representative component of ginseng rare saponin and can protect against multiple organs, also used as functional food to adjust the balance of the human body, but the therapeutic effect and molecular mechanism of G-Rg3 on male diseases under HS are underexplored. The aim of the present study, G-Rg3 was prepared through the efficient conversion of ginsenoside Rd and investigate the contribution of G-Rg3 to testicular injury induced exposure to HS. All mice were divided into four groups as… More >

HONGWEI CHEN^#, XUAN SONG^#, HEMEI LI^*

BIOCELL, Vol.44, No.3, pp. 345-351, 2020, DOI:10.32604/biocell.2020.08944

Abstract Ovarian cancer (OC) is a major cause of cancer-related deaths among gynaecological malignancies. Emerging studies suggest that the long non-coding RNA (lncRNA) may be the potential biomarker for the diagnosis and prognosis of the cancer. The current study was carried out to investigate the role of lncRNA CCHE1 silencing in OC cell invasion and migration. Expression of lncRNA CCHE1 in normal ovarian cell Hose and OC cell lines HO 8910, A2780 and SKOV3 was detected. LncRNA were transfected with siRNA, and then the proliferation of cells was detected by using MTT assay. Cell invasion and migration was measured by using… More >