SHAHID KARIM^1,*, ALANOUD NAHER ALGHANMI¹, MAHA JAMAL¹, HUDA ALKREATHY¹, ALAM JAMAL², HIND A. ALKHATABI³, MOHAMMED BAZUHAIR¹, AFTAB AHMAD^4,5

Oncology Research, Vol.32, No.5, pp. 817-830, 2024, DOI:10.32604/or.2024.048988

Abstract Cancer frequently develops resistance to the majority of chemotherapy treatments. This study aimed to examine the synergistic cytotoxic and antitumor effects of SGLT2 inhibitors, specifically Canagliflozin (CAN), Dapagliflozin (DAP), Empagliflozin (EMP), and Doxorubicin (DOX), using in vitro experimentation. The precise combination of CAN+DOX has been found to greatly enhance the cytotoxic effects of doxorubicin (DOX) in MCF-7 cells. Interestingly, it was shown that cancer cells exhibit an increased demand for glucose and ATP in order to support their growth. Notably, when these medications were combined with DOX, there was a considerable inhibition of glucose consumption, as well as reductions in… More > Graphic Abstract

ZEINAB KLAAB¹, AZIZA HASSAN², JAWAHER ALBAQAMI¹, FAIZAH A. ALMALKI^1,*

BIOCELL, Vol.47, No.4, pp. 891-904, 2023, DOI:10.32604/biocell.2023.026556

Abstract Cancer remains to be one of the most severe sicknesses globally. Cases have kept rising over the years. Breast cancer (BC), which is among the leading types of cancers and predominantly affects women, is the second leading cause of cancer mortality. Researchers have developed interventions over the years; however, the BC survival rate has not improved since the 1980s. This has created the need for novel drug interventions that would manage and treat BC more effectively. This study focused on using a combination of natural product extracts such as phytoestrogen (Ziziphus jujube) and Tannin nanoparticles (NP99) together, which we have… More >

Çağrı Öner^1,* and Ertuğrul Çolak²

Oncologie, Vol.23, No.3, pp. 393-407, 2021, DOI:10.32604/Oncologie.2021.016958

Abstract piRNAs are novel members of small non-coding RNAs and have an impact on genetic and epigenetic mechanisms of cells. It was aimed to investigate the role of piR-651 on MCF-7 benign breast cancer cells by focusing on molecular characteristics. Anti-piR-651 was transfected and effects of piR-651 on proliferation, adhesion, and motility of MCF-7 cells were detected after the 24th, 48th, and 72nd hour. Gene expressions of piR-651, Ki-67, MMP-2, ERα, HIF-1α, and hTERT were determined by using RT-PCR. piR-651 inhibition caused the decrease of proliferation, adhesion (p < 0.001), and motility of MCF-7 cells. The efficiency of anti-piR-651 transfection supported… More >

Faizah A. AlMalki¹, Aziza M. Hassan^2,*, Zeinab M. Klaab¹, Soliman Abdulla³, Antonio Pizzi⁴

Journal of Renewable Materials, Vol.9, No.12, pp. 2077-2092, 2021, DOI:10.32604/jrm.2021.016173

Abstract Recently, natural substances in the form of nanoparticles are increasingly being used in different field, particularly in medicines to enhance their beneficial effects in treatment and prevention. Cancer cells of the breast (MCF-7) have been chosen to be examined and treated in vitro with conventional drug Tamoxifen (Tam) and tannin nanoparticles extract (NP99) individually or in combination. MTT reagent has been applied to assess the cell viability and propagation percentage, DNA fragmentation and mRNA relative expression of apoptotic genes to study the cell death pathway. The results showed that Tam and tannin NP99 triggered cytotoxic activity towards the MCF-7 cell.… More >

Kena Lv¹, Shuangshuang Zheng², Xiangqin Li¹, Yi Nie^2,3,*, Tianqing Liu¹, Kedong Song^1,*

Molecular & Cellular Biomechanics, Vol.17, No.4, pp. 199-204, 2020, DOI:10.32604/mcb.2020.011811

Abstract As one of the adjuvant treatments for cancer treatment, traditional Chinese medicine treatment has a wide range of cancer treatments, such as preventing metastasis and relapse, improving the efficacy of radiotherapy and chemotherapy, reducing the side effects of chemotherapy, improving body function, extending life and improving the life quality. Geniposide (GEN) is a bioactive substance extracted from the fruit of gardenia. In recent years, it has attracted attention due to its anti-tumor effect. In this study, we aimed to investigate whether GEN could inhibit the proliferation of human breast cancer cells (MCF-7) and promote its apoptosis. The half-inhibitory concentration (IC50)… More >

CHRIS VORSTER, ANNIE JOUBERT

BIOCELL, Vol.34, No.2, pp. 71-80, 2010, DOI:10.32604/biocell.2010.34.071

Abstract In the search for new and improved anticancer therapies, researchers have identified several potentially useful compounds. One of these agents is 2-methoxyestradiol-bis-sulphamate (2ME-BM), a sulphamoylated derivative of 2-methoxyestradiol. The objective of this study was to evaluate 2ME-BM’s in vitro efficacy as antiproliferative agent in the MCF-7 breast adenocarcinoma cell line. Light- and fluorescent microscopy showed decreased cell density, increased apoptotic characteristics and significant ultrastructural aberrations indicative of autophagic cell death after 24 hours of exposure at a concentration of 0.4μM. In addition, mitotic indices revealed that 2ME-BM induces a G₂M block. The latter was confirmed by flow cytometric analyses where… More >

ANNIE JOUBERT^1*, SUMARI MARAIS¹, CHRISTINE MARITZ²

BIOCELL, Vol.33, No.1, pp. 67-70, 2009, DOI:10.32604/biocell.2009.33.067

Abstract Proteins of the B-cell lymphoma 2 family are crucial for the regulation of apoptosis. B-cell lymphoma 2-associated X is a pro-apoptotic protein, while B-cell lymphoma 2 protein opposes apoptosis. The influence of 1 μM 2-methoxyestradiol was investigated on the expression levels of these two proteins in MCF-7 cells. 2-Methoxyestradiol exposure did not influence B-cell lymphoma 2 protein expression levels after 24 h of exposure. In contrast, B-cell lymphoma 2-associated X protein levels were significantly reduced. An improved differential interference contrasting technique revealed compromised cell density and the presence of a mitotic block in exposed cells. The study proposes that the… More >

SARA SOLTANIAN¹, HELIA RIAHIRAD¹, ATHAREH PABARJA¹, MOHAMMAD REZA KARIMZADEH², KOLSOUM SAEIDI^3,4

BIOCELL, Vol.41, No.2-3, pp. 33-40, 2017, DOI:10.32604/biocell.2017.41.033

Abstract Cancer stem cells (CSCs) are a small subpopulation of cancer cells whose resistance to chemotherapy and radiotherapy plays a pivotal role in treatment failure, tumor recurrence and metastasis. Today, the ability of many phytochemicals in targeting of CSCs has been identified. Kaempferol is a plant phytoestrogen that was recognized as a useful agent for targeting cancer cells by apoptosis induction, cell cycle arrest and inhibition of angiogenesis, migration, and invasion of cancer cells. In this study, we compared the effect of docetaxel as a common breast cancer chemotherapy drug and kaempferol on MCF-7 breast CSCs. Results showed that both docetaxel… More >

Moharam Habibnejad Korayem^1,*, Zahra Rastegar²

Molecular & Cellular Biomechanics, Vol.16, No.2, pp. 109-122, 2019, DOI:10.32604/mcb.2019.04706

Abstract The mechanical properties of single cells have been recently identified as the basis of an emerging approach in medical applications since they are closely related to the biological processes of cells and human health conditions. The problem in hand is how to measure mechanical properties in order to obtain them more accurately and applicably. Some of the cell’s properties such as elasticity module and adhesion have been measured before using various methods; nevertheless, comprehensive tests for two healthy and cancerous cells have not been performed simultaneously. As a Nanoscale device, AFM has been used for some biological cells, however for… More >