WEI ZHAI1,2, XIANG ZHENG2, MENGYUAN LIU2, QIURONG ZHAO2, YUSHU ZHANG2, YUQING LUO3, SHANSHAN FENG3, JINJING WANG3, XUEYING LI2,*, MINGSONG WU1,*
BIOCELL, Vol.47, No.8, pp. 1821-1833, 2023, DOI:10.32604/biocell.2023.027175
Abstract Objective: Endoplasmic reticulum-Golgi intermediate compartment 3 (ERGIC3) promotes cell proliferation and metastasis in lung cancer, but its molecular mechanism is unclear. Methods: The GLC-82 cells were randomly divided into the ERGIC3i group and the negative control group. The cells were transfected with ERGIC3 siRNA or control siRNA in the groups, respectively. The ERGIC3-interacting proteins expressed in cells or extracellularly were isolated by the immunoprecipitation method and detected by isobaric tags for relative and absolute quantitation and liquid chromatography-tandem mass spectrometry. The differentially expressed proteins were determined by bioinformatic methods. Results: After ERGIC3 knockdown, 88 extracellular differentially expressed proteins, 41 up-regulated… More >
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