JING LI1,2, WANWAN FAN4, LILI HAO1, YONGSHENG LI5, GUOCHENG YU1, WEI SUN6, XIANQIONG LUO2,*, JINGXIANG ZHONG1,3,*
BIOCELL, Vol.47, No.11, pp. 2485-2494, 2023, DOI:10.32604/biocell.2023.044177
Abstract Objective: This study aimed to investigate the potential of human umbilical cord mesenchymal stem cell
(hucMSC)-derived exosomes (hucMSC-Exos) in inhibiting hypoxia-induced cell hyper proliferation and
overexpression of vascular endothelial growth factor A (VEGF-A) in immature human fetal retinal microvascular
endothelial cells (hfRMECs). Methods: Exosomes were isolated from hucMSCs using cryogenic ultracentrifugation
and characterized through various techniques, including transmission electron microscopy, nanoparticle tracking
analysis, bicinchoninic acid assays, and western blotting. The hfRMECs were identified using von Willebrand factor
(vWF) co-staining and divided into four groups: a control group cultured under normoxic condition, a hypoxic
model group, a hypoxic group treated with… More >
Graphic Abstract
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