ABDELAZIZ ADAM IDRISS ARBAB1,3,#, CHUNQING YIN4,#, XUBIN LU1, YAN LIANG1, ISMAIL MOHAMED ABDALLA1, AMER ADAM IDRIS3, TIANLE XU1,2, YONGJIANG MAO1, ZHANGPING YANG1,2,*
BIOCELL, Vol.46, No.11, pp. 2443-2454, 2022, DOI:10.32604/biocell.2022.020865
Abstract Mastitis is a common inflammatory cow mammary infection; that causes significant economic loss in dairy
industry. Given the interesting connection between metformin’s anti-inflammatory function and mastitis model
induced by LTA in pbMECs, our objective was to prove that metformin was beneficial in suppressing
proinflammatory response induced by LTA through modulation of mitogen-activated protein kinase (MAPK) and
nuclear factor kappa B (NF-κB) signaling pathways and activation of peroxisome proliferator-activated receptor-γ
(PPARγ) in pbMECs. The proliferation of cells and mRNA expression were measured using EdU assay and
quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Immunoblotting and immunofluorescence
analysis were conducted to evaluate the… More >
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