HUIZE CHEN1,2,*, XINGTIAN JIA1,2, HAIYAN RAN1,2, JINGRONG NIU1,2, MEITING DU1
BIOCELL, Vol.46, No.2, pp. 559-565, 2022, DOI:10.32604/biocell.2022.017437
Abstract As an allohexaploid plant, common wheat has a complex gene structure, making it difficult to study its gene function. Virus-induced gene silencing (VIGS) is an important tool for the rapid analysis of plant gene function. In this study, the gene silencing system, namely, barley stripe mosaic virus (BSMV)-VIGS induced by BSMV was used to silence the wheat phytoene desaturase (TaPDS) and actin depolymerization factor (TaADF7) genes and determine the effect of gene silencing on wheat. TaPDS was used as an indicator gene to determine the feasibility of VIGS system, while TaADF7 was used as a test gene to determine its… More >
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