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    Molecular and Cellular Immuno-Engineering

    Yingxiao Wang1,*

    Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 79-79, 2019, DOI:10.32604/mcb.2019.07268

    Abstract Genetically-encoded biosensors based on fluorescence proteins (FPs) and fluorescence resonance energy transfer (FRET) have enabled the specific targeting and visualization of signaling events in live cells with high spatiotemporal resolutions. Single-molecule FRET biosensors have been successfully developed to monitor the activity of variety of signaling molecules, including tyrosine/serine/threonine kinases. We have a developed a general high-throughput screening (HTS) method based on directed evolution to develop sensitive and specific FRET biosensors. We have first applied a yeast library and screened for a mutated binding domain for phosphorylated peptide sequence. When this mutated binding domain and the peptide sequence are connected by… More >

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