Escalante-González JG1, EA García-Zambrano2, A Gutiérrez-Diez2, RE Vázquez-Alvarado2, JA Torres-Castillo2, SR Sinagawa-García2, F Zavala-García2, J Martínez-de la Cerda2, C Gallegos-Vázquez3, JI García-Zambrano4
Phyton-International Journal of Experimental Botany, Vol.81, pp. 143-148, 2012, DOI:10.32604/phyton.2012.81.143
Abstract The objective of this research was to increase the genetic diversity of the Nopal Germplasm Bank of the FAUANL using RAPD molecular markers to estimate the genetic diversity of 15 accessions of cactus pear from molecular markers recorded in the Bank. DNA extraction was performed by the method of cellular ruptor using FastDNA® Kit. Performance measurement of DNA was carried out through Picoflour. It was observed that the DNA concentrations ranged from 74.91 ng/µL to 12.32 ng/uL. Twelve primers were used to generate from 1 to 5 bands per primer with a molecular weight ranging from 200 to 1000 bp.… More >
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