Joshua A. Zimmermann*, Melissa L. Knothe Tate∗,†,‡
Molecular & Cellular Biomechanics, Vol.8, No.4, pp. 275-296, 2011, DOI:10.3970/mcb.2011.008.275
Abstract Shape and fate are intrinsic manifestations of form and function at the cell scale. Here we hypothesize that seeding density and protocol affect the form and function of live embryonic murine mesenchymal stem cells (MSCs) and their nuclei. First, the imperative for study of live cells was demonstrated in studies showing changes in cell nucleus shape that were attributable to fixation per se. Hence, we compared live cell and nuclear volume and shape between groups of a model MSC line (C3H10T1/2) seeded at, or proliferated from 5,000 cells/cm2 to one of three target densities to achieve targeted development contexts. Cell… More >
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