HILDA L. MONTRULL, NILDA Y. BRIZUELA, SILVIA L. DEMURTAS, LUIS SPITALE* , CARLOS I. MEIROVICH.
BIOCELL, Vol.29, No.2, pp. 163-167, 2005, DOI:10.32604/biocell.2005.29.163
Abstract Cartilage samples were taken from OA patients in order to describe and quantify pro-inflammatory mediators. Samples were cultured under aseptic conditions in Dulbecco’s modified Eagle medium at 37°C for 10 days. Control samples, taken from non-inflammatory cartilage, were cultured under the same conditions. The levels of NO-2 and NO-3 were measured in the supernatant using a spectrophotometric assay. The activity of MMP-1 was quantified by ELISA.
The concentration of NO-x was 47.3 ± 4.1 µM in the OA cartilague and 10.7 ± 1.8 µM in the controls. The average MMP-1 activity was 3,650 ± 387 ng/ml in the OA… More >
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