JIRUI WEN1,#, WENCHAO WU2,#, MIN TANG1, MINGYUE BAO1, XUELING HE3, XINGHONG YAO1, LIANG LI1,*
BIOCELL, Vol.46, No.3, pp. 655-665, 2022, DOI:10.32604/biocell.2022.016915
Abstract To dissect which subset of bone marrow monocyte is the major precursor of osteoclast, 3-month-old rat bone marrow was obtained for single-cell RNA sequencing. A total of 6091 cells were acquired for detailed analysis, with a median number of 1206 genes detected per cell and 17,959 genes detected in total. A total of 19 cell clusters were recognized, with the main lineages identified as B cells, Granulocytes, Monocytes, T cells, Erythrocytes and Macrophages. Monocytes were further divided into classical monocytes and non-classical monocytes. Compared with non-classical monocytes, classical monocytes highly expressed osteoclast differentiation related genes Mitf, Spi1, Fos and Csf1r.… More >
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