TING ZHOU^1,#, PEIYING FU^1,#, DONG CHEN², RONGHUA LIU^1,*

Oncology Research, Vol.31, No.5, pp. 769-785, 2023, DOI:10.32604/or.2023.030166

Abstract Late-stage ovarian cancer (OC) has a poor prognosis and a high metastasis rate, but the underlying molecular mechanism is unclear. RNA binding proteins (RBPs) play important roles in posttranscriptional regulation in the contexts of neoplasia and tumor metastasis. In this study, we explored the molecular functions of a canonical RBP, Transformer 2β homolog (TRA2B), in cancer cells. TRA2B knockdown in HeLa cells and subsequent whole-transcriptome RNA sequencing (RNA-seq) analysis revealed the TRA2B-regulated alternative splicing (AS) profile. We disrupted TRA2B expression in epithelial OC cells and performed a series of experiments to confirm the resulting effects on OC cell proliferation, apoptosis… More >

TIANMING SHI^1,2,#, RONGRONG YAN^1,2,#, MI HAN^1,2,*

Oncology Research, Vol.31, No.4, pp. 591-604, 2023, DOI:10.32604/or.2023.029282

Abstract Ovarian cancer (OV) is highly heterogeneous tumor with a very poor prognosis. Studies increasingly show that T cell exhaustion is prognostically relevant in OV. The aim of this study was to dissect the heterogeneity of T cell subclusters in OV through single cell transcriptomic analysis. The single RNA-sequencing (scRNA-seq) data of five OV patients were analyzed, and six major cell clusters were identified after threshold screening. Further clustering of T cell-associated clusters revealed four subtypes. Pathways related to oxidative phosphorylation, G2M checkpoint, JAK-STAT and MAPK signaling were significantly activated, while the p53 pathway was inhibited in the CD8+ exhausted T… More > Graphic Abstract

YAPING GAN^1,2,#, TING LIU^3,#, WEIFENG FENG^1,#, LIANG WANG⁴, LI LI⁵, YINGXIA NING^1,*

Oncology Research, Vol.31, No.3, pp. 333-343, 2023, DOI:10.32604/or.2023.028694

Abstract Various therapeutic strategies have been developed to overcome ovarian cancer. However, the prognoses resulting from these strategies are still unclear. In the present work, we screened 54 small molecule compounds approved by the FDA to identify novel agents that could inhibit the viability of human epithelial ovarian cancer cells. Among these, we identified disulfiram (DSF), an old alcohol-abuse drug, as a potential inducer of cell death in ovarian cancer. Mechanistically, DSF treatment significantly reduced the expression of the anti-apoptosis marker B-cell lymphoma/leukemia-2 (Bcl-2) and increase the expression of the apoptotic molecules Bcl2 associated X (Bax) and cleaved caspase-3 to promote… More >

QINGLING TANG¹, WARDA ATIQ², SHAISTA MAHNOOR², MOSTAFA A. ABDEL-MAKSOUD³, MOHAMMED AUFY⁴, HAMID YAZ^3,*, JIANYU ZHU^5,*

Oncology Research, Vol.31, No.2, pp. 141-156, 2023, DOI:10.32604/or.2023.028548

Abstract Though significant improvements have been made in the treatment methods for ovarian cancer (OC), the prognosis for OC patients is still poor. Exploring hub genes associated with the development of OC and utilizing them as appropriate potential biomarkers or therapeutic targets is highly valuable. In this study, the differentially expressed genes (DEGs) were identified from an independent GSE69428 Gene Expression Omnibus (GEO) dataset between OC and control samples. The DEGs were processed to construct the protein-protein interaction (PPI) network using STRING. Later, hub genes were identified through Cytohubba analysis of the Cytoscape. Expression and survival profiling of the hub genes… More >

YANPING JIN¹, JIANPING QIU¹, XIUFANG LU¹, YAN MA¹, GUOWEI LI^2,*

Oncology Research, Vol.31, No.2, pp. 169-179, 2023, DOI:10.32604/or.2023.027815

Abstract Previous study revealed that ferritin heavy chain-1 (FTH1) could regulate ferritinophagy and affect intracellular Fe²⁺ content in various tumors, while its N6-methyladenosine (m6A) RNA methylation was closely related the prognosis of ovarian cancer patients. However, little is known about the role of FTH1 m6A methylation in ovarian cancer (OC) and its possible action mechanisms. In this study we constructed FTH1 m6A methylation regulatory pathway (LncRNA CACNA1G-AS1/IGF2BP1) according to related bioinformatics analysis and research, through clinical sample detections we found that these pathway regulatory factors were significantly up-regulated in ovarian cancer tissues, and their expression levels were closely related to the… More >

QIAOLING WU^1,2, ZHAOLEI CUI³, HONGMEI XIA¹, SHAN JIANG¹, JING BAI⁴, ZHUO SHAO⁴, YANG SUN^1,*

BIOCELL, Vol.47, No.5, pp. 1039-1050, 2023, DOI:10.32604/biocell.2023.027494

Abstract Background: Ovarian cancer (OC) is a leading cause of gynecological cancer-linked deaths worldwide. Exosomal miR-1825 and its target gene C-type lectin domain family 5 member A (CLEC5A) are associated with tumorigenesis in cancers that was further probed. Methods: Exosomal miR-1825 expression in exosomes and its impact on overall survival (OS) prediction were determined using Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) data. Target genes of miR-1825 were searched in five prediction databases and prognostically significant differentially expressed genes were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were carried out. The… More >

KAMILA CZUBAK-PROWIZOR^*, MARIA SWIATKOWSKA

BIOCELL, Vol.47, No.4, pp. 731-737, 2023, DOI:10.32604/biocell.2023.025677

Abstract Junctional adhesion molecule-A (JAM-A), also known as the F11 receptor (F11R), is one of the tight junction components. JAM-A is a transmembrane glycoprotein that regulates many cellular processes, i.e., angiogenesis, leukocyte transendothelial migration, intercellular permeability, epithelial-to-mesenchymal transition, and platelet activation. Of note, it is involved in the pathogenesis of various cancer types, including gynecological cancers. Only a few studies are available about this cancer type. Observed aberrant JAM-A expression in gynecological cancers correlates with poor patient prognosis. To the best of our knowledge, conflicting JAM-A roles in various cancer types suggest that its involvement is complex and tumor-type specific. The… More >

LIJUN DU¹, KAIKAI DOU¹, NIANHAI LIANG^1,2, JIANMIN SUN¹, RU BAI^1,*

BIOCELL, Vol.47, No.3, pp. 547-554, 2023, DOI:10.32604/biocell.2023.025048

Abstract Background: The Warburg effect is considered as a hallmark of various types of cancers, while the regulatory mechanism is poorly understood. Our previous study demonstrated that miR-194-5p directly targets and regulates insulin-like growth factor1 receptor (IGF1R). In this study, we aimed to investigate the role of miR-194-5p in the regulation of the Warburg effect in ovarian cancer cells. Methods: The stable ovarian cell lines with miR-194-5p overexpression or silencing IGF1R expression were established by lentivirus infection. ATP generation, glucose uptake, lactate production and extracellular acidification rate (ECAR) assay were used to analyze the effects of aerobic glycolysis in ovarian cancer… More >

Chaoying Liu^1,#, Xinfeng Yang^2,#, Ye Wang^2,#, Keyu Wu², Siqiang Li², Gailing Wang², Yun Li², Chuanfeng Li², Mingcheng Wang², Enzhong Li^2,*

Oncologie, Vol.24, No.4, pp. 679-691, 2022, DOI:10.32604/oncologie.2022.025705

Abstract A cell is the basic unit of life, and death is inevitable for any cell. However, cancer cells that deviate from the normal track can resist death and survive. Ferroptosis is recently discovered as a modulated cell death different from other known forms of cell death in morphology, biochemistry, and genetics. It is characterized by iron-dependent lipid peroxidation regulated by various metabolic pathways. The incidence and mortality of genitourinary system cancer have been increasing recently. Although clinical practice therapy techniques have improved, no plan with a positive prognosis has been identified. For the therapy of cancer, ferroptosis opens up new… More >

HAN WANG, YINGYING ZHOU, SIYANG ZHANG, YA QI, MIN WANG^*

Oncology Research, Vol.29, No.4, pp. 275-289, 2021, DOI:10.32604/or.2022.03561

Abstract Metastasis and paclitaxel (PTX) resistance are the main reason for the poor prognosis of ovarian cancer (OC). Evidence showed that RNA-binding proteins (RBPs) and long noncoding RNAs (lncRNAs) can modulate post-transcriptional regulation. The aim of this study was to determine the relationship among RBP, lncRNA and OC and to further guide clinical therapy. Immunohistochemistry revealed that pre-mRNA processing factor 6 (PRPF6) was upregulated in OC chemoresistant tissues and was closely related to advanced (Federation of International of Gynecologists and Obstetricians) FIGO stages and chemo-resistance. PRPF6 promoted progression, and PTX resistance in vitro and in vivo. And the transcripts of small… More >