Heya QIAN1,§, Yan YAN2,§, Zhengjie SHEN1, Lixian XU1, Yun ZUO1, Tao ZHU3,*, Yanan CHEN1,*
BIOCELL, Vol.43, No.4, pp. 321-326, 2019, DOI:10.32604/biocell.2019.07992
Abstract The present study aims to explore the effects of p53 and its target gene Rap2B on the autophagy of U2OS
cells. U2OS cells were treated with siRNA against p53, Rap2B, and PLCε. Relative expressions of p53, Rap2B, and PLCε
were determined using quantitative polymerase chain reaction (qPCR) and Western blotting, respectively. Levels of IP3
in the cells were determined using Enzyme-linked Immunosorbent Assay (ELISA). Levels of Ca2+ were detected using Flow cytometry. Fluorescence microscopy was used to observe the autophagy of cells. Knockdown of p53 significantly
decreased the expressions of Rap2B protein. Additionally, knockdown of p53 significantly decreased the mRNA… More >
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