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  • Open Access

    ARTICLE

    Nucleotide Sequence Assessment of Four ORFs of Citrus Tristeza Virus: Evidence of Recombination

    Adel A. Rezk1,2,*, Hala A. Amin2

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 691-705, 2023, DOI:10.32604/phyton.2022.024208

    Abstract Citrus Tristeza Virus (CTV), usually occurs in nature as a mixture of genotypes. Six naturally infected citrus (Citrus sinensis) trees grafted on sour orange rootstock were collected from three citrus growing governorates in Egypt (Sharqia, Qalyubia and Garbia). In this study, RT-PCR, Single-Strand Conformation Polymorphism (SSCP) and nucleotide sequence analysis were used for four independent CTV genomic regions (p65, p18, p20, and p23) to detect and assess the sequence and genetic variabilities among CTV Egyptian isolates. RTPCR products (650 bp) for the CTV p23 gene obtained from the selected isolates were used for the SSCP analysis and DNA sequencing. SSCP… More >

  • Open Access

    ARTICLE

    Morphological and Phylogenetic Resolution of Diplodia neojuniperi Emerging Diplodia Top Dieback of Pinus thunbergii Parl. in China

    Xiaolei Ding, Ruiwen Zhao, Sixi Lin, Jianren Ye*

    Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2813-2825, 2022, DOI:10.32604/phyton.2022.024609

    Abstract In Bazhong City, Sichuan Province, China, top dieback symptoms were found on many pine trees (Pinus thunbergii Parl). The tips of old needles first turned grayish-green and then developed into brown bands in the field. Phylogenetic analysis of concatenated ITS and EF1-α indicated the pathogen of this dieback disease as Diplodia neojuniperi. Additionally, effects of temperature, pH and medium on the mycelial growth were also characterized. The most favorable temperature and pH level for mycelial growth are 25°C and 8, respectively. The optimal medium for mycelial growth is PDA medium. To our knowledge, this is the first report of D.… More >

  • Open Access

    COMMUNICATION

    Electrochemical Identification of Yulania spp. by Fingerprinting of Leaves Using Glassy Carbon Electrode

    Zhiguo Lu1, Yuhong Zheng1,*, Pengchong Zhang2, Boyuan Fan3, Aimin Yu4, Li Fu3,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.11, pp. 2549-2558, 2022, DOI:10.32604/phyton.2022.021288

    Abstract In this communication, we used electrochemical sensor for recording the electrochemical profiles of eleven species of Yulania spp. from leaf extract. Two solvents and two buffer conditions were used for electrochemical fingerprints collection. Their electrochemical fingerprints can be converted to different patterns and consequently for species recognition. The results indicate the pattern recognition is much convenient than that of the recognition of species directly using voltammetric signal. The current information in electrochemical fingerprinting represents the type and amount of electrochemically active molecules, which linked to the genetic differences among the plants. Therefore, the electrochemical fingerprints were applied for further phylogenetic… More >

  • Open Access

    ARTICLE

    Insight into the characteristics of an important evolutionary model bird (Geospiza magnirostris) mitochondrial genome through comparison

    ZHENGGANG XU1,2,3, LIANG WU3, JIAHAO CHEN1, YUNLIN ZHAO3, CHONGXUAN HAN1, TIAN HUANG2, GUIYAN YANG1,*

    BIOCELL, Vol.46, No.7, pp. 1733-1746, 2022, DOI:10.32604/biocell.2022.015784

    Abstract Darwin’s finches are the most classic case of evolution. Early studies on the evolution of this species were mainly based on morphology. Until now, the mitochondrial genome of Geospiza magnirostris has been sequenced and the study explored the characteristics of the complete genome of G. magnirostris and verified the evolutionary position of it. The 13 PCGs initiated by ATN codons. The stop codons of three PCGs (ND2, COX3 and ND4) were incomplete, with only T- or TA- replacing complete form TAA or TAG. All the tRNA genes expressed a typical cloverleaf secondary structure, except for tRNASer1(AGY), whose dihydrouridine (DHU) arm… More >

  • Open Access

    ARTICLE

    Phylogenetic analysis and target gene prediction of miR477 gene family in grape

    HUI-YING JIN1,2, MAO-SONG PEI1,2, DA-LONG GUO1,2,*

    BIOCELL, Vol.46, No.4, pp. 941-949, 2022, DOI:10.32604/biocell.2022.016718

    Abstract To understand the molecular characteristics of the miR477 gene family of grape (Vvi-miR477) and to predict its target genes, the Vvi-miR477 genes were identified from previous small RNA sequencing data, then phylogenetic analysis and prediction of target gene were conducted. The Vvi-miR477 family consists of two precursor sequences and three mature sequences. The miR477 family members were mostly 19-22nt in length. The sequence is relatively conservative. Vvi-MIR477a and Vvi-MIR477b are located on chromosomes 1 and 2, respectively. These precursor sequences can form the typical stable stem-loop structure. Their minimum folding free energy is −39.10 kcal/mol and −50.90 kcal/mol, respectively. The… More >

  • Open Access

    ARTICLE

    Overexpression of a Glycosyltransferase Gene from a Metabolically Poly-Resistant Beckmannia syzigachne Population Alters Growth and Confers Herbicide Resistance to Brachypodium distachyon

    Yizhao Huang1, Nannan Li2, Deya Wang4, Jian Du1, Weitang Liu3, Jinxin Wang3,*, Wei Li1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 761-772, 2022, DOI:10.32604/phyton.2022.018462

    Abstract Beckmannia syzigachne is a noxious weed for rice-wheat rotations in China. The B. syzigachne (AH-02) population evolved metabolic resistance to fenoxaprop-P-ethyl and mesosulfuron-methyl. To investigate the function of GT73C1 in this population, the GT73C1 gene was amplified by reverse transcription-polymerase chain reaction, and the sequence was 100% consistent with the transcriptome data. Its phylogenetic tree was displayed and annotated using FigTree v1.4.4. The plant overexpression vector of GT73C1 gene was constructed and used to transform Brachypodium distachyon plants. Furthermore, the expression of GT73C1 was significantly induced by fenoxaprop-P-ethyl and mesosulfuron-methyl, which was consistent with the findings from the whole plant… More >

  • Open Access

    ARTICLE

    The chloroplast genome comparative characteristic of artificial breeding tree, a case about Broussonetia kazinoki × Broussonetia papyrifera

    WAN ZHANG1,2,#, GUIYAN YANG3,#, YUNLIN ZHAO1, ZHENGGANG XU1,2,3,*, HUANG HUIMIN1, JIAKANG ZHOU1

    BIOCELL, Vol.46, No.3, pp. 803-819, 2022, DOI:10.32604/biocell.2022.016984

    Abstract Broussonetia kazinoki × Broussonetia papyrifera (ZJGS) is a hybrid species in Moraceae family, which has a very complicated hybrid origin. The excellent characteristics of fast growth, strong soil and water conservation ability, high leaf protein content and stem fiber content in ZJGS make it both ecological benefits in the mining area and economically valuable. This study aims to further understand ZJGS and other Moraceae taxa through the ZJGS chloroplast (cp) genome structure and the comparison with 12 closely related Moraceae species. Among the 13 Moraceae species, the cp genome length of seven Broussonetia species (ranges from 160,239 bp to 162,594… More >

  • Open Access

    ARTICLE

    Complete genome sequence of two strawberry vein banding virus isolates from China

    LEI JIANG1,2,3,#, SHUAI LI1,#, XIZI JIANG1, TONG JIANG1,2,3,*

    BIOCELL, Vol.45, No.6, pp. 1695-1702, 2021, DOI:10.32604/biocell.2021.015250

    Abstract It was rarely reported about strawberry vein banding virus (SVBV) genome sequence in China and most countries worldwide. In this work, we determined the complete genome sequences of two SVBV isolates in China, designated SVBV-AH and SVBV-BJ, that were obtained from naturally infected strawberry samples from Anhui province and Beijing city of China, respectively. The complete genomes of SVBV-AH and SVBV-BJ were 7,862 nucleotides (nts) and 7,863 nts long, respectively, and both constituted with seven genes typical of the caulimoviruses. Alignment of complete nucleotide sequences showed that SVBV-AH and SVBV-BJ shared a significant nucleotide sequence identity of 97.7% of each… More >

  • Open Access

    ARTICLE

    Phylogenetic analysis of microRNA biomarkers for amyotrophic lateral sclerosis

    HSIUYING WANG1,2,*

    BIOCELL, Vol.45, No.3, pp. 547-561, 2021, DOI:10.32604/biocell.2021.014343

    Abstract Amyotrophic lateral sclerosis (ALS), also called Lou Gehrig’s disease, is an irreversible disease that is caused by the degeneration and death of motor neurons. Approximately 5–10% of cases are familial ALS (fALS), and the other cases are sporadic ALS (sALS). Gene mutations have been identified both in fALS and sALS patients. In this study, we discuss the four ALS-related genes, C9orf72, SOD1, FUS, and TARDBP, and review the microRNAs (miRNAs) that are associated with ALS and other neurological disorders from the literature. A phylogenetic analysis is used to explore potential miRNAs that can be taken into account when studying the… More >

  • Open Access

    ARTICLE

    Comparative Analyses and Phylogenetic Relationships between Cryptomeria fortunei and Related Species Based on Complete Chloroplast Genomes

    Yingting Zhang1,2,3, Junjie Yang1,2,3, Zhenhao Guo1,2,3, Jiaxing Mo1,2,3, Jiebing Cui1,2,3, Hailiang Hu1,2,3, Jin Xu1,2,3,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 957-986, 2020, DOI:10.32604/phyton.2020.011211

    Abstract Cryptomeria fortunei (Chinese cedar) is a highly adaptable woody species and one of the main forest plantation trees in subtropical high-altitude areas in China. However, there are few studies on its chloroplast (cp) genome. In this study, the complete cp genome of C. fortunei was sequenced and evaluated via comparative analyses with those of related species (formerly the Taxodiaceae) in Cupressaceae. The C. fortunei cp genome was 131,580 bp in length, and the GC content of the whole genome was 35.38%. It lost one relevant large inverted repeat and contained 114 unique genes, including 82 protein-coding genes, 28 tRNAs and… More >

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