Wentao Sheng^1,2,*

Phyton-International Journal of Experimental Botany, Vol.94, No.7, pp. 2015-2031, 2025, DOI:10.32604/phyton.2025.066232 - 31 July 2025

Abstract Rorippa indica is a wild oilseed crop of Brassicaceae with good environmental adaptability and strong stress resistance. This plant has become an important wild relative species for rapeseed (Brassica napus L.) and is used to improve its agronomic traits, with important development and utilization value. However, the research of R. indica genetics is still lacking. And no mitochondrial genome (mitogenome) in the genus Rorippa has been expounded. To analyze the structural characteristics of the R. indica mitogenome, second-generation and third-generation sequencing techniques were made to assemble its mitogenome. The results showed that its mitogenome is composed of a single master… More >

Zichen Li^1,#, Jukui Ma^2,#, Minjun Liu³, Guowei Geng^1,*, Hongxia Zhang^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.6, pp. 1841-1856, 2025, DOI:10.32604/phyton.2025.066148 - 27 June 2025

Abstract Sweet potatoes are significant cash crops, however, their yield and quality are greatly compromised by viral diseases. In this study, the complete genomic sequences of two Sweet Potato Virus 2 (SPV2) isolates from infected sweet potato leaves in the Shandong (designated as SPV2-SDYT, GenBank No. PQ855660.1) and Jiangsu (designated as SPV2-JSXZ, GenBank No. PQ855661.1) provinces in China were obtained using 5^′ RACE and RT-PCR amplification. Consistency, phylogeny, codon usage bias, recombination, and selection pressure analyses were conducted using the SPV2-SDYT and SPV2-JSXZ genome sequences. The complete genome sequences of SPV2-SDYT and SPV2-JSXZ were 10561 nucleotides (nt)… More >

Ahmed Mahmoud Ismail^1,2,*, Khalid Alhudaib¹, Donato Magistà^3,4

Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 679-693, 2025, DOI:10.32604/phyton.2025.062196 - 31 March 2025

Abstract Coffee wilt represents one of the most devastating diseases of Arabica coffee (Coffea arabica L.) plantations in the primary coffee-producing regions. In this study, coffee trees manifesting wilt symptoms accompanied by the defoliation and drying of the whole tree were observed in the Jazan, El Baha, Najran, and Asir regions. The purpose of this investigation was to isolate and identify the Fusarium species recovered from symptomatic coffee trees. The developed fungi were initially characterized based on their morphological features followed by molecular phylogenetic multi-locus analysis of the combined sequences of ITS, TEF1-α, RPB2, and CaM. Twenty-five isolates… More >

Wentao Sheng^*

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 331-345, 2025, DOI:10.32604/phyton.2025.061424 - 06 March 2025

Abstract Pyrola atropurpurea Franch is an important annual herbaceous plant. Few genomic analyses have been conducted on this plant, and chloroplast genome research will enrich its genomics basis. This study is based on high-throughput sequencing technology and Bioinformatics methods to obtain the sequence, structure, and other characteristics of the P. atropurpurea chloroplast genome. The result showed that the chloroplast genome of P. atropurpurea has a double-stranded circular structure with a total length of 172,535 bp and a typical four-segment structure. The genome has annotated a total of 132 functional genes, including 43 tRNAs, 8 rRNAs, 76 protein-coding genes, and… More >

Wentao Sheng, Xi Lei, Xinjie Chen, Quan Kuang^*

Phyton-International Journal of Experimental Botany, Vol.93, No.8, pp. 1897-1911, 2024, DOI:10.32604/phyton.2024.052395 - 30 August 2024

Abstract The analysis of chloroplast gene characteristics in Alpinia japonica (Thunb.) Miq. is of great significance for developing relevant genetic resources. The high-throughput sequencing and bioinformatic research were performed to analyze the chloroplast genome characteristics of A. japonica. The total chloroplast genome length of A. japonica was 161,906 bp, with a typical circular tetrameric structure. And 133 genes were annotated, comprising 87 protein-coding, 38 tRNA, and 8 rRNA genes. Furthermore, 22 genes contained two copies, and 18 genes owned introns. Repeat sequence analysis showed that it contains 321 simple sequence repeats (SSRs) and 37 long segment repeats. Compared with… More >

Chaoying Wang¹, Lihui Ma², Yingzan Xie², Bo Ding³, Jingsi Huang⁴, Qian Wang^4,*

Phyton-International Journal of Experimental Botany, Vol.93, No.8, pp. 1981-1993, 2024, DOI:10.32604/phyton.2024.050099 - 30 August 2024

Abstract Myricaria laxiflora, which grows along the Yangtze River in China, holds ornamental, ecological, and medicinal value. However, its wild population is threatened and currently designated protected as a national priority. The present research was the first to sequence and assemble M. laxiflora’s mitochondrial genome and examine its structural characteristics and phylogenetic relationships with other sequenced Caryophyllales species. The mitochondrial double-stranded closed-ring genome of M. laxiflora was found to be 389,949 bp in length, containing numerous repetitive sequences and RNA editing sites, with 34 protein encoding, 21 tRNA, and 3 rRNA genes. Although there are 22 fragments in the More >

Riwa Mahai¹, Rongpeng Liu¹, Xiaolang Du¹, Zejing Mu¹, Xiaoyun Wang^1,*, Jun Yuan^2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.6, pp. 1203-1219, 2024, DOI:10.32604/phyton.2024.051559 - 27 June 2024

Abstract Rosaceae represents a vast and complex group of species, with its classification being intricate and contentious. The taxonomic placement of many species within this family has been a subject of ongoing debate. The study utilized the Illumina platform to sequence 19 plant species from 10 genera in the Rosaceae. The cp genomes, varying in size from 153,366 to 159,895 bp, followed the typical quadripartite organization consisting of a large single-copy (LSC) region (84,545 to 87,883 bp), a small single-copy (SSC) region (18,174 to 19,259 bp), and a pair of inverted repeat (IR) regions (25,310 to… More >

Zhengfeng Lai, Dongmei Lian, Shaoping Zhang, Yudong Ju, Bizhen Lin, Yunfa Yao, Songhai Wu, Jianji Hong, Zhou Li^*

Phyton-International Journal of Experimental Botany, Vol.93, No.6, pp. 1143-1158, 2024, DOI:10.32604/phyton.2024.051207 - 27 June 2024

Abstract The cytokinin oxidase/dehydrogenase (CKX) enzyme is essential for controlling the fluctuating levels of endogenous cytokinin (CK) and has a significant impact on different aspects of plant growth and development. Nonetheless, there is limited knowledge about CKX genes in tomato (Solanum lycopersicum L.). Here we performed genome-wide identification and analysis of nine SlCKX family members in tomatoes using bioinformatics tools. The results revealed that nine SlCKX genes were unevenly distributed on five chromosomes (Chr.1, Chr.4, Chr.8, Chr.10, and Chr.12). The amino acid length, isoelectric points, and molecular weight of the nine SlCKX proteins ranged from 453 to 553, 5.77… More >

Adel A. Rezk^1,2,*, Hala A. Amin²

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 691-705, 2023, DOI:10.32604/phyton.2022.024208 - 29 November 2022

Abstract Citrus Tristeza Virus (CTV), usually occurs in nature as a mixture of genotypes. Six naturally infected citrus (Citrus sinensis) trees grafted on sour orange rootstock were collected from three citrus growing governorates in Egypt (Sharqia, Qalyubia and Garbia). In this study, RT-PCR, Single-Strand Conformation Polymorphism (SSCP) and nucleotide sequence analysis were used for four independent CTV genomic regions (p65, p18, p20, and p23) to detect and assess the sequence and genetic variabilities among CTV Egyptian isolates. RTPCR products (650 bp) for the CTV p23 gene obtained from the selected isolates were used for the SSCP analysis… More >

Xiaolei Ding, Ruiwen Zhao, Sixi Lin, Jianren Ye^*

Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2813-2825, 2022, DOI:10.32604/phyton.2022.024609 - 29 August 2022

Abstract In Bazhong City, Sichuan Province, China, top dieback symptoms were found on many pine trees (Pinus thunbergii Parl). The tips of old needles first turned grayish-green and then developed into brown bands in the field. Phylogenetic analysis of concatenated ITS and EF1-α indicated the pathogen of this dieback disease as Diplodia neojuniperi. Additionally, effects of temperature, pH and medium on the mycelial growth were also characterized. The most favorable temperature and pH level for mycelial growth are 25°C and 8, respectively. The optimal medium for mycelial growth is PDA medium. To our knowledge, this is the first More >