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  • Open Access

    ARTICLE

    Nucleotide Sequence Assessment of Four ORFs of Citrus Tristeza Virus: Evidence of Recombination

    Adel A. Rezk1,2,*, Hala A. Amin2

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 691-705, 2023, DOI:10.32604/phyton.2022.024208

    Abstract Citrus Tristeza Virus (CTV), usually occurs in nature as a mixture of genotypes. Six naturally infected citrus (Citrus sinensis) trees grafted on sour orange rootstock were collected from three citrus growing governorates in Egypt (Sharqia, Qalyubia and Garbia). In this study, RT-PCR, Single-Strand Conformation Polymorphism (SSCP) and nucleotide sequence analysis were used for four independent CTV genomic regions (p65, p18, p20, and p23) to detect and assess the sequence and genetic variabilities among CTV Egyptian isolates. RTPCR products (650 bp) for the CTV p23 gene obtained from the selected isolates were used for the SSCP analysis and DNA sequencing. SSCP… More >

  • Open Access

    ARTICLE

    Insight into the characteristics of an important evolutionary model bird (Geospiza magnirostris) mitochondrial genome through comparison

    ZHENGGANG XU1,2,3, LIANG WU3, JIAHAO CHEN1, YUNLIN ZHAO3, CHONGXUAN HAN1, TIAN HUANG2, GUIYAN YANG1,*

    BIOCELL, Vol.46, No.7, pp. 1733-1746, 2022, DOI:10.32604/biocell.2022.015784

    Abstract Darwin’s finches are the most classic case of evolution. Early studies on the evolution of this species were mainly based on morphology. Until now, the mitochondrial genome of Geospiza magnirostris has been sequenced and the study explored the characteristics of the complete genome of G. magnirostris and verified the evolutionary position of it. The 13 PCGs initiated by ATN codons. The stop codons of three PCGs (ND2, COX3 and ND4) were incomplete, with only T- or TA- replacing complete form TAA or TAG. All the tRNA genes expressed a typical cloverleaf secondary structure, except for tRNASer1(AGY), whose dihydrouridine (DHU) arm… More >

  • Open Access

    ARTICLE

    The chloroplast genome comparative characteristic of artificial breeding tree, a case about Broussonetia kazinoki × Broussonetia papyrifera

    WAN ZHANG1,2,#, GUIYAN YANG3,#, YUNLIN ZHAO1, ZHENGGANG XU1,2,3,*, HUANG HUIMIN1, JIAKANG ZHOU1

    BIOCELL, Vol.46, No.3, pp. 803-819, 2022, DOI:10.32604/biocell.2022.016984

    Abstract Broussonetia kazinoki × Broussonetia papyrifera (ZJGS) is a hybrid species in Moraceae family, which has a very complicated hybrid origin. The excellent characteristics of fast growth, strong soil and water conservation ability, high leaf protein content and stem fiber content in ZJGS make it both ecological benefits in the mining area and economically valuable. This study aims to further understand ZJGS and other Moraceae taxa through the ZJGS chloroplast (cp) genome structure and the comparison with 12 closely related Moraceae species. Among the 13 Moraceae species, the cp genome length of seven Broussonetia species (ranges from 160,239 bp to 162,594… More >

  • Open Access

    ARTICLE

    Comparative Analyses and Phylogenetic Relationships between Cryptomeria fortunei and Related Species Based on Complete Chloroplast Genomes

    Yingting Zhang1,2,3, Junjie Yang1,2,3, Zhenhao Guo1,2,3, Jiaxing Mo1,2,3, Jiebing Cui1,2,3, Hailiang Hu1,2,3, Jin Xu1,2,3,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 957-986, 2020, DOI:10.32604/phyton.2020.011211

    Abstract Cryptomeria fortunei (Chinese cedar) is a highly adaptable woody species and one of the main forest plantation trees in subtropical high-altitude areas in China. However, there are few studies on its chloroplast (cp) genome. In this study, the complete cp genome of C. fortunei was sequenced and evaluated via comparative analyses with those of related species (formerly the Taxodiaceae) in Cupressaceae. The C. fortunei cp genome was 131,580 bp in length, and the GC content of the whole genome was 35.38%. It lost one relevant large inverted repeat and contained 114 unique genes, including 82 protein-coding genes, 28 tRNAs and… More >

  • Open Access

    ARTICLE

    Comparative fine structure of the epididymal spermatozoa from three korean shrews with considerations on their phylogenetic relationships

    SOON-JEONG JEONG1, JOO-CHEOL PARK1, HEUNG-JOONG KIM2, CHUN SIK BAE3, MYUNG-HEE YOON4, DO-SEON LIM5, MOON-JIN JEONG1

    BIOCELL, Vol.30, No.2, pp. 279-286, 2006, DOI:10.32604/biocell.2006.30.279

    Abstract This study examined the fine structures of epididymal spermatozoa on the lesser white-toothed shrew (Crocidura suaveolens), the Japanese white-toothed shrew (C. dsinezumi) and the big white-toothed shrew (C. lasiura) belonging to the subfamily Crocidurinae living in Korea.
    In the spermatozoa of C. suaveolens, the head has a large acrosome, a smooth inner acrosomal membrane and a wavy, finger-like, electron-dense apical body. The neck has a solid proximal centriole that is filled with electron-dense material. These results showed the spermatozoa of C. suaveolens possess the characteristics of both Crocidurinae and Soricinae. In C. dsinezumi and C. lasiura, the head has… More >

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