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    ARTICLE

    Biosynthesis of raw starch degrading β-cyclodextrin glycosyltransferase by immobilized cells of Bacillus licheniformis using potato wastewater

    YASSER S. MOSTAFA1,*, SAAD A. ALAMRI1,2, SULIMAN A. ALRUMMAN1, TAREK H. TAHA3, MOHAMED HASHEM1,4, MAHMOUD MOUSTAFA1,5, LAMIAA I. FAHMY6

    BIOCELL, Vol.45, No.6, pp. 1661-1672, 2021, DOI:10.32604/biocell.2021.016193 - 01 September 2021

    Abstract The study was sought to enhance the synthesis of thermal stable β-cyclodextrin glycosyltransferase (β-CGTase) using potato wastewater as a low-cost medium and assess the degree to which it is efficient for industrial production of β-cyclodextrin (β-CD) from raw potato starch. Thermophilic bacteria producing β-CGTase was isolated from Saudi Arabia and the promising strain was identified as Bacillus licheniformis using phylogenetic analysis of the 16S rRNA gene. Alginate-encapsulated cultures exhibited twice-fold of β-CGTase production more than free cells. Scanning electron microscopy (SEM) of polymeric capsules indicated the potential for a longer shelf-life, which promotes the restoration of… More >

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