YASSER S. MOSTAFA1,*, SAAD A. ALAMRI1,2, SULIMAN A. ALRUMMAN1, TAREK H. TAHA3, MOHAMED HASHEM1,4, MAHMOUD MOUSTAFA1,5, LAMIAA I. FAHMY6
BIOCELL, Vol.45, No.6, pp. 1661-1672, 2021, DOI:10.32604/biocell.2021.016193
Abstract The study was sought to enhance the synthesis of thermal stable β-cyclodextrin glycosyltransferase (β-CGTase)
using potato wastewater as a low-cost medium and assess the degree to which it is efficient for industrial production of
β-cyclodextrin (β-CD) from raw potato starch. Thermophilic bacteria producing β-CGTase was isolated from Saudi
Arabia and the promising strain was identified as Bacillus licheniformis using phylogenetic analysis of the 16S rRNA
gene. Alginate-encapsulated cultures exhibited twice-fold of β-CGTase production more than free cells. Scanning
electron microscopy (SEM) of polymeric capsules indicated the potential for a longer shelf-life, which promotes the
restoration of activity in bacterial cells… More >
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