FENG ZHU*, QINQIN ZHANG, YANGKAI ZHOU, QIPING ZHANG, MENGYAO CAO, ZHAOLIN JI
BIOCELL, Vol.46, No.9, pp. 2123-2131, 2022, DOI:10.32604/biocell.2022.020229
Abstract The NAC (NAM, ATAF, and CUC) superfamily is one of the largest plant-specific families containing transcription factors. An increasing number of studies suggest that NAC1 is involved in plants response to different biotic and abiotic stimulis. Nicotiana benthamiana is a widely used system for evaluating plant-pathogen interactions. In order to study the biochemical function of NbNAC1, NbNAC1 protein and antibody are essential. Therefore, we focused on developing a prokaryotic expression system for producing the Nicotiana benthamiana NbNAC1 protein of in Escherichia coli and the preparation of its polyclonal antibody. Firstly, we constructed two different molecular weight prokaryotic expression vectors: pGE… More >
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