YANFEI LI1, LULU DAI2, KE CAI2, YINGKUI SONG2, XIQING LIU3,*
BIOCELL, Vol.45, No.3, pp. 685-694, 2021, DOI:10.32604/biocell.2021.013612
Abstract RPA3 (Replication Protein A3) (14 kD) is a part of the canonical heterotrimeric replication protein A complex
(RPA/RP-A). This study aimed to explore the functional role of RPA3 and the mechanisms of its dysregulation in breast
cancer. Data from the Cancer Genome Atlas (TCGA)-breast cancer patients and GSE75688 were utilized for gene
expression and survival analysis. Breast cancer cell lines MDA-MB-231 and SK-BR-3 were used for in-vitro cell
studies. Clonogenic assay and immunofluorescent staining of γ-H2AX were performed to examine radiation-induced
cytotoxicity. Systemic correlation analysis was performed to identify potential transcription factors (TFs) regulating
RPA3 expression. ChIP-qPCR and dual-luciferase assay… More >
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