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  • Open Access

    ARTICLE

    Mapping of the Pepper Purple Fruit Gene and Development of Molecular Markers Based on BSA-Seq

    Xinxin Liu1,#, Yueyue Zhang1,#, Rahat Sharif1, Weiqin Mo2, Zhenrong Li1, Guiling Yang1, Chao Song1, Ting Ye2,*, Changming Chen1,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.6, pp. 1695-1709, 2025, DOI:10.32604/phyton.2025.063349 - 27 June 2025

    Abstract The color difference of capsicum fruit is closely related to the type and content of pigment in the peel, which is mainly determined by anthocyanins, chlorophyll, and carotenoids. This study used green “CA59” and purple “Z81” pepper fruits as parents to create the F2 generation. The fruit color of 466 F2 population was identified, and the extreme individuals from this population were selected for Bulked Segregant Analysis (BSA) using resequencing. Genetic analysis revealed that a pair of genes controls the expression of the purple fruit trait in capsicum. Using functional annotation, expression analysis, and sequencing analysis… More >

  • Open Access

    ARTICLE

    Interplay of Temporal Variation in Nectar Parameters and Pollinator-Mediated Adaptations in Epimedium wushanense

    Lanying Chen1,2, Yifu Cai3, Qiumei Quan3,*, Yunxiang Li3

    Phyton-International Journal of Experimental Botany, Vol.94, No.5, pp. 1519-1532, 2025, DOI:10.32604/phyton.2025.064112 - 29 May 2025

    Abstract This study investigates the diurnal patterns of nectar secretion, sugar content, and caloric value in Epimedium wushanense, and their interaction mechanisms with pollinator behavior under varying environmental conditions. Nectar secretion exhibited a diurnal pattern, peaking between 11:00 and 13:00, with progressive increases in both volumes (19.07 ± 1.66 μL/day) and caloric value (6.03 ± 0.55 cal/day) over four consecutive days, culminating in maximal production on Day 4 (p < 0.05). Floral bagging significantly altered nectar traits (Mann-Whitney U test, p < 0.05), with bagged inflorescences demonstrating 61.82% higher nectar volume productivity relative to unbagged controls. Pollinator visitation,… More >

  • Open Access

    ARTICLE

    The new gold standard for surgical management of BPH: an institutional experience with 1000 HoLEPs

    Yash B. Shah1, Brian H. Im1, Aaron R. Hochberg1, Elliott P. Freudenburg2, James Jiang2, Bruce M. Gao2, Mihir S. Shah1, Akhil K. Das2,*

    Canadian Journal of Urology, Vol.32, No.1, pp. 15-19, 2025, DOI:10.32604/cju.2025.064708 - 20 March 2025

    Abstract Introduction: Holmium Laser Enucleation of the Prostate (HoLEP) is a size-independent, endoscopic management option for benign prostatic hyperplasia (BPH). HoLEP offers a distinct advantage for patients who are at high-risk for bleeding whilst preserving prostatic tissue for pathology analysis, unlike photoselective vaporization. Further, HoLEP avoids the need for cystotomy, unlike simple open and robotic prostatectomy, by using intravesical morcellation. We report our experience with the first 1000 HoLEP procedures at our institution. Materials and Methods: We performed a retrospective review of all HoLEP procedures performed at our institution from 2013–2021 to capture patient demographics, procedure details,… More >

  • Open Access

    ARTICLE

    Utilizing Fine-Tuning of Large Language Models for Generating Synthetic Payloads: Enhancing Web Application Cybersecurity through Innovative Penetration Testing Techniques

    Stefan Ćirković1, Vladimir Mladenović1, Siniša Tomić2, Dalibor Drljača2, Olga Ristić1,*

    CMC-Computers, Materials & Continua, Vol.82, No.3, pp. 4409-4430, 2025, DOI:10.32604/cmc.2025.059696 - 06 March 2025

    Abstract With the increasing use of web applications, challenges in the field of cybersecurity are becoming more complex. This paper explores the application of fine-tuned large language models (LLMs) for the automatic generation of synthetic attacks, including XSS (Cross-Site Scripting), SQL Injections, and Command Injections. A web application has been developed that allows penetration testers to quickly generate high-quality payloads without the need for in-depth knowledge of artificial intelligence. The fine-tuned language model demonstrates the capability to produce synthetic payloads that closely resemble real-world attacks. This approach not only improves the model’s precision and dependability but… More >

  • Open Access

    ARTICLE

    A Novel Reliable and Trust Objective Function for RPL-Based IoT Routing Protocol

    Mariam A. Alotaibi1,2,*, Sami S. Alwakeel1,*, Aasem N. Alyahya1

    CMC-Computers, Materials & Continua, Vol.82, No.2, pp. 3467-3497, 2025, DOI:10.32604/cmc.2025.060599 - 17 February 2025

    Abstract The Internet of Things (IoT) integrates diverse devices into the Internet infrastructure, including sensors, meters, and wearable devices. Designing efficient IoT networks with these heterogeneous devices requires the selection of appropriate routing protocols, which is crucial for maintaining high Quality of Service (QoS). The Internet Engineering Task Force’s Routing Over Low Power and Lossy Networks (IETF ROLL) working group developed the IPv6 Routing Protocol for Low Power and Lossy Networks (RPL) to meet these needs. While the initial RPL standard focused on single-metric route selection, ongoing research explores enhancing RPL by incorporating multiple routing metrics… More >

  • Open Access

    HOW I DO IT

    How I Do It: Teaching holmium laser enucleation of the prostate (HoLEP)

    Agustín Pérez-Londoño, Alejandro Abello, Boris Gershman, Ruslan Korets

    Canadian Journal of Urology, Vol.31, No.2, pp. 11848-11853, 2024

    Abstract Holmium laser enucleation of the prostate (HoLEP) is considered a size-independent technique to treat benign prostatic hyperplasia. This safe and effective procedure is increasingly being adopted in urology training programs worldwide, yet limited teaching strategies have been described. Endoscopic handling during HoLEP allows for a simultaneous interaction between the surgeon and trainee, facilitating a guided teaching strategy with increasing difficulty as experience grows. In this article, we describe our stepwise approach for teaching HoLEP as part of a structured surgical training curriculum. We also evaluate the association of our method with intraoperative efficiency parameters and More >

  • Open Access

    HOW I DO IT

    How I Do It: Holmium laser cystolitholapaxy and enucleation of the prostate for benign prostatic hyperplasia

    Bruce M. Gao, Seyedamirvala Saadat, Edward J. H. Choi, James Jiang, Akhil K. Das

    Canadian Journal of Urology, Vol.31, No.3, pp. 11904-11907, 2024

    Abstract Holmium enucleation of the prostate (HoLEP) is a gold standard, size-independent surgical treatment for benign prostatic hyperplasia (BPH) distinguished for its efficacy in tissue removal, shorter catheterization durations, lower transfusion rates, and decreased hospital stays when compared to transurethral resection of the prostate (TURP). The objective of this article is to demonstrate the step-by-step procedure of holmium laser cystolitholapaxy and enucleation of the prostate for BPH, emphasizing a top-down modified two-lobe technique with early apical release which enhances visualization and irrigation flow during the enucleation process. More >

  • Open Access

    COMMENTARY

    A commentary on the interplay of biomaterials and cell adhesion: new insights in bone tissue regeneration

    A. NOEL GRAVINA1,2, NOELIA D´ELÍA1,2, LUCIANO A. BENEDINI2,3,*, PAULA MESSINA1,2

    BIOCELL, Vol.48, No.11, pp. 1517-1520, 2024, DOI:10.32604/biocell.2024.055513 - 07 November 2024

    Abstract This article navigates the relationship between biomaterials and osteogenic cell adhesion, highlighting the importance of mimicking the physiological response for bone tissue regeneration. Within this spirit is an initial description of the interaction between osteoblasts and osteoprogenitor cells with the extracellular matrix, explaining the leading role of integrins and cadherins in cell adhesion, and the intracellular signaling pathways elicited. Additionally, there is a focus on the strategies of advanced biomaterials that foster osteogenesis by replicating the native environment, taking advantage of these known specific signaling pathways. The final remarks lay on the need for careful More >

  • Open Access

    PROCEEDINGS

    In-Situ Monitoring of Interplay Between Melt Pool, Spatter and Vapor in Laser Powder Bed Fusion Additive Manufacturing

    Xin Lin1,2,3, Kunpeng Zhu1,2,3,*

    The International Conference on Computational & Experimental Engineering and Sciences, Vol.30, No.2, pp. 1-1, 2024, DOI:10.32604/icces.2024.012499

    Abstract This paper reveals the interplay mechanism between melt pool, spattering and vapors, aiming to further improve the forming quality through in-situ monitoring with a CMOS camera. A Residual Network based on Convolutional Block Attention Module and Focal loss function is proposed to extract multi-scale features of single tracks and learn about their behavior changes. A t-SNE clustering analysis is utilized to analysis a large amount of time sequence data on the melt pool by collecting the schlieren photographs. It is found that patterns of unstable melt pool changing corelate to the defects in single tracks, More >

  • Open Access

    MINI REVIEW

    Interplay between Plants and Microbial Communities: Insights from Holobionts and Environmental Interactions

    Sejin Choi1,2, Ho-Seok Lee1,2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.10, pp. 2519-2534, 2024, DOI:10.32604/phyton.2024.058012 - 30 October 2024

    Abstract Plants interact with a complex network of microorganisms, forming a dynamic holobiont that is crucial for their health, growth, and adaptation. This interconnected system is deeply influenced by environmental factors, which modulate the relationships within the plant microbiome. Key environmental drivers such as light, temperature, and moisture can alter the balance of these interactions, impacting plant immunity, resilience, and overall fitness. The traditional disease triangle model, which emphasizes plant-pathogen-environment interactions, is enhanced by incorporating the role of the microbiome, revealing how microbial communities contribute to disease outcomes. This review highlights the importance of shifting focus More >

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