Lu Zhu, Qiuyue Ma, Shushun Li, Jing Wen, Kunyuan Yan, Qianzhong Li^*

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 385-403, 2020, DOI:10.32604/phyton.2020.09259

Abstract Quantitative real-time reverse transcriptase PCR (qRT-PCR) technology has been extensively used to estimate gene expression levels, and the selection of appropriate reference genes for qRT-PCR analysis is critically important for obtaining authentic normalized data. Acer palmatum is an important colorful leaf ornamental tree species, and reference genes suitable for normalization of the qRT-PCR data obtained from this species have not been investigated. In this study, the expression stability of ten candidate reference genes, namely, Actin3, Actin6, Actin9, EF1α, PP2A, SAMDC, TIP41, TUBα, TUBβ and UBQ10, in two distinct tissues (leaves and roots) of A. palmatum under four different abiotic stress… More >

Gang Li¹, Yao Zhou², Yaqi Zhao², Yaxue Liu², Yuwei Ke², Xiaoqing Jin¹, Haoli Ma^1,2,*

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 329-344, 2020, DOI:10.32604/phyton.2020.08874

Abstract Quantitative real-time PCR (qRT-PCR) is widely used for investigating gene expression patterns and has many advantages, including its high sensitivity, fidelity, and specificity. Selecting a satisfactory internal reference gene is crucial for obtaining precise gene expression results in qRT-PCR analyses. In this study, the transcriptomic data of 2 potato varieties were screened for housekeeping genes with stable expression patterns. A total of 77 putative genes were selected, which were highly and stably expressed. Then, qRT-PCR analyses were performed to examine the expression levels of these 77 candidate reference genes in various potato tissues, including leaves, flowers, stolons, and tubers. Gene… More >

ANDREA P. RODRIGUEZ, HIDETSUGU TSUJIGIWA, MEHMET GUNDUZ, BEYHAN CENGIZ, NORIYUKI NAGAI, RYO TAMAMURA, SILVIA S. BORKOSKY, TOHRU TAKAGI, MIHO INOUE, HITOSHI NAGATSUKA*

BIOCELL, Vol.33, No.1, pp. 39-47, 2009, DOI:10.32604/biocell.2009.33.039

Abstract Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed… More >

JOEL ARNEODO*, SOLEDAD DE BREUIL*, SERGIO LENARDON**, LUIS CONCI**

BIOCELL, Vol.29, No.2, pp. 205-207, 2005, DOI:10.32604/biocell.2005.29.205

Abstract Plants of Viola cornuta displaying typical virus symptoms were observed during spring 2003 in a plant nursery in Córdoba, central Argentina. Electron microscopic examinations of symptomatic leaf samples revealed the presence of isometric virus-like particles about 30 nm in diameter. Subsequent serological analysis allowed the identification of the pathogen as a subgroup I strain of Cucumber mosaic virus (CMV). These results were confirmed by antigen capture - reverse transcription - polymerase chain reaction with specific CMV primers, and digestion with a restriction enzyme. This is the first report of CMV infecting V. cornuta in Argentina. More >

Ocampo Ocampo T¹, DL Ochoa Martínez¹, S Ramírez Rojas², G Valdovinos Ponce¹, C Nava Díaz¹

Phyton-International Journal of Experimental Botany, Vol.82, pp. 235-241, 2013, DOI:10.32604/phyton.2013.82.235

Abstract The Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), in Zacatepec Morelos, started a genetic improvement program of wild and semi-cultivated poinsettia plants ("nochebuena de sol"). It is important to generate a phytosanitary database that allows the release of new varieties and hybrids that provide tolerance to the major pathogens reported in cultivated poinsettia plants. The phytosanitary status of the “nochebuena de sol” plants is not available in Mexico, so the objective of this research was to generate preliminary data about viruses associated to these materials. Based on DAS-ELISA and RT-PCR results, we reported the presence of the Tobacco… More >

Gonzalo RODRÍGUEZ-LOMBARDI¹, Luciana REYNA², María Susana VITALI¹, Susana GENTI-RAIMONDI²

BIOCELL, Vol.39, No.2-3, pp. 15-24, 2015, DOI:10.32604/biocell.2015.39.015

Abstract A competitive internal control (IC) adapted to RT-PCR in-house assay was developed for HCV RNA detection in human pooled plasma. Also, it was applied in a multiplex RT-PCR for the HIV-1 and HCV RNA screening in human pooled plasma and plasma-derived products. A 258-bp PCR product from the 5´noncoding region of HCV genome was obtained. A competitive IC template was constructed by inserting a 52-bp double strand sequence into the NheI site of the 258-bp amplicon. This sequence was cloned and the obtained plasmid was used to generate a synthetic RNA. The IC/RNA was incorporated in in-house HCV and/or HIV… More >