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  • Open Access

    ARTICLE

    Variability of Metarhizium anisopliae var. anisopliae based on the Random Amplified Polymorphic DNA technique

    García-Pereyra J1, GN Aviña-Martínez1, AA Orozco-Flores2, OG Alvarado-Gómez3, M García-Montelongo4, G Alejandre-Iturbide5, JN Uribe-Soto5, H Medrano-Roldan6

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 37-43, 2014, DOI:10.32604/phyton.2014.83.037

    Abstract It was analyzed the genetic variability of (1) three strains of a pathogenic fungi of the genus Metharhizium anisopliae var anisopliae, named MA22, MA24 and MA25, brand spesifik®; (2) two strains of the product Metasaven®, named MA1A and MAII; one strain of the product Metatron®; and a native strain named MACN. This latter strain was directly isolated from the insect chapulin (Brachystola magna), from the farmer, corn plots in Durango, located in north central Mexico. Studies were conducted in the laboratory of molecular biology of the Technological Institute of the Valley of Guardiana in Durango, Mexico, from September 2008 to… More >

  • Open Access

    ARTICLE

    Genetic diversity of water primrose (Ludwigia hyssopifolia) in Thailand based on morphological characters and RAPD analysis

    Tantasawat PA, K Lunwongsa, T Linthaisong, P Wirikitgul, N Campatong, N Talpolkrung, A Tharapreuksapong, O Poolsawat, A Khairum, A Sorntip, C Kativat

    Phyton-International Journal of Experimental Botany, Vol.87, pp. 162-170, 2018, DOI:10.32604/phyton.2018.87.162

    Abstract Genetic diversity and relatedness of 17 water primrose (Ludwigia hyssopifolia) accessions in Thailand were estimated using morphological characters and random amplified polymorphic DNA (RAPD) markers. Eight morphological characters were diverse among the accessions. However, some accessions could not be distinguished from one another based on these morphological characters alone. Unweighted pair-group arithmetic average (UPGMA) analysis of these characters separated these 17 accessions into 2 major clusters. Among the 5 RAPD primers used, a total of 68 fragments (150 to 2000 bp) were amplified, showing a polymorphism percentage of 80%. The polymorphic information content (PIC) among accessions varied from 0.31 to… More >

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