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    Immune strategies of phagoctytic cells stimulated in vitro with live and heat-inactivated Streptococcus pyogenes

    Agustín César MÖHLINGERa, Virginia Paula SIFFREDIa, Serenela CHULIBERTa, Adriana PONESSAb, Rodolfo David NOTARIOb, Diana Graciela DLUGOVITZKYa

    BIOCELL, Vol.37, No.3, pp. 63-69, 2013, DOI:10.32604/biocell.2013.37.063

    Abstract Streptococcus pyogenes (group A Streptococcus) is frequently involved in a wide range of human diseases. Here we evaluated polymorphonuclear neutrophils and mononuclear cells from healthy subjects for their bactericidal function after stimulation with live and inactivated Streptococcus pyogenes (Streptococcus Group A). Mononuclear cells and Neutrophils were isolated from heparinized blood samples (n=18) using a Ficoll-Hypaque gradient and cultured in RPMI 1640 for 18 hours with a suspension of either live or inactivated Streptococcus pyogenes. Both the respiratory burst (flow cytometry) and nitrite, TNF and IL17 production (ELISA) were measured in the cell culture supernatants. An increased respiratory burst (expressed as… More >

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