Agustín César MÖHLINGERa, Virginia Paula SIFFREDIa, Serenela CHULIBERTa, Adriana PONESSAb, Rodolfo David NOTARIOb, Diana Graciela DLUGOVITZKYa
BIOCELL, Vol.37, No.3, pp. 63-69, 2013, DOI:10.32604/biocell.2013.37.063
Abstract Streptococcus pyogenes (group A Streptococcus) is frequently involved in a wide range of human diseases. Here we evaluated polymorphonuclear neutrophils and mononuclear cells from healthy subjects for their bactericidal function after stimulation with live and inactivated Streptococcus pyogenes (Streptococcus Group A). Mononuclear cells and Neutrophils were isolated from heparinized blood samples (n=18) using a Ficoll-Hypaque gradient and cultured in RPMI 1640 for 18 hours with a suspension of either live or inactivated Streptococcus pyogenes. Both the respiratory burst (flow cytometry) and nitrite, TNF and IL17 production (ELISA) were measured in the cell culture supernatants. An increased respiratory burst (expressed as… More >
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