Feng1 RJ, LF Lu2, KH Yuan3, P Cheng3, LL Zhang3, JF Qi3, Y Ren4, XL Xu5, XB Zhang3, LY Zhou3, YD Zhang3
Phyton-International Journal of Experimental Botany, Vol.79, pp. 163-168, 2010, DOI:10.32604/phyton.2010.79.163
Abstract A banana (Musa AAA, Cavendish subgroup cv. Brazil) cDNA encoding a putative rubredoxin-like protein (MaRd1) was obtained from total RNA isolated from cold-treated banana leaves using rapid amplification of cDNA ends (RACE) technique. MaRd1 cDNA contained 597 nucleotides encoding 198 amino acids in the open reading frame. MaRd1 protein showed 56% amino acid identity with that of Pyrococcus furiosus rubredoxin (P24297). A chloroplast transit peptide and a transmembrane region were detected at the N-terminus and the C-terminus, respectively, of the deduced amino acid sequence of MaRd1 gene. Southern blotting revealed the occurrence of at least two copies of MaRd1 in… More >
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