Dandan Wang, Xiaohong Li, Yanwen Zhang^*

Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 355-369, 2024, DOI:10.32604/phyton.2024.047183

Abstract Taxus cuspidata is a rare plant with important medicinal and ornamental value. Aiming at the obvious differences between wild and cultivated populations of T. cuspidata from Northeast China, a total of 61 samples, that is, 33 wild yews and 28 cultivated yews were used to analyze the differences and correlations of the kinship, genetic diversity, and genetic structure between them by specific length amplified fragment sequencing (SLAF-seq). Finally, 470725 polymorphic SLAF tags and 58622 valid SNP markers were obtained. Phylogenetic analysis showed that 61 samples were classified into 2 clusters: wild populations and cultivated populations, and some wild yews were… More >

Fen Zhang¹, Weili Tian¹, Lu Cen¹, Litang Lv², Xiaofang Zeng¹, Yulu Chen¹, Yichen Zhao^2,*, Yan Li^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 791-809, 2022, DOI:10.32604/phyton.2022.018104

Abstract Duyun Maojian tea is a famous tea in China. In this study, the specific-locus amplified fragment (SLAF) sequencing method was used to analyze the population structure and conduct a genome-wide association study (GWAS) of 2 leaf traits of 123 tea plants in Qiannan, China. A total of 462,019 SLAF tags and 11,362,041 single-nucleotide polymorphism (SNP) loci were obtained. The results of phylogenetic tree analysis, cluster analysis, and principal component analysis showed that 123 tea germplasms were clustered into three groups, and the heterozygosity rates for Groups I, II, and II were 0.206, 0.224, and 0.34, respectively. Generally, tea germplasms in… More >

Sumei Li, Qing Wang, Peng Wang, Shuan Wang, Rutong Yang, Ya Li^*

Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 617-632, 2022, DOI:10.32604/phyton.2022.017741

Abstract Carpinus oblongifolia is an endemic species and the extant wild populations show a fragmentation distribution in the Baohua Mountain of Jiangsu Province in eastern China. Understanding of genetic diversity plays an important role in C. oblongifolia survival and sustainable development. The wild C. oblongifolia population was artificially divided into four subpopulations according to the microhabitats, and another two subpopulations were constructed by progeny seedlings cultivated with the mature seeds. Then, the leaf buds of 80 individuals from six subpopulations were sampled to develop single nucleotide polymorphisms (SNPs) using specific-locus amplified fragment sequencing (SLAF-seq). Based on these SNPs, we aimed to… More >