ANA CECILIA LIAUDAT, FRANCISCA EBEL, BIANCA ANA OPIZZO, MARINA AURORA GONZALEZ, DAMIÁN BLOIS, PABLO BOSCH#, NANCY RODRIGUEZ#,*
BIOCELL, Vol.47, No.5, pp. 1135-1143, 2023, DOI:10.32604/biocell.2023.027628
Abstract Background: Capacitation is a set of physiological changes sperms undergo to acquire fertilizing capacity. In vivo, this process is directly associated with high calcium levels in sperm cytoplasm. Calcitriol, the vitamin D hypercalcemic metabolite, is related to human sperm motility, capacitation, and acrosome reaction. This work aimed to study the effect of calcitriol on bull sperm quality parameters and capacitation. Methods: One million freeze-thawed spermatozoa were obtained from different bulls and treated with 20 nM of calcitriol for 30 min. Untreated cells (negative control) and treated ones with calcitriol or heparin (100 µg/mL, positive capacitation control) were evaluated for motility,… More >
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