MENGMENG WU^1,#, PENG GUO^1,#, YISU SHI^1,#, DANYAN ZHENG¹, QIAONAN ZHANG¹, XIN JIN¹, YUHUA WANG¹, QIANG LIN², RONGJUN FANG^1,*, WEIGUO ZHAO^1,*

BIOCELL, Vol.46, No.8, pp. 1935-1945, 2022, DOI:10.32604/biocell.2022.019223

Abstract A variety of plants were colchicine treated to double their chromosome number. Chromosomes are genetic carriers that determine the individual traits of organisms. The doubling of chromosomes will lead to modifications in plant morphology, physiology and genetics. To determine the response of mulberry trees induced by colchicine, using mulberry variety Yu-711 leaves as research materials, two small RNA libraries (control and experimental groups) were constructed. It was found that 45 known miRNA genes and 78 predicted novel miRNA genes in the sequence results. A comparison of data between the control group and the experimental group revealed 37 differentially expressed miRNA… More >

HUI-YING JIN^1,2, MAO-SONG PEI^1,2, DA-LONG GUO^1,2,*

BIOCELL, Vol.46, No.4, pp. 941-949, 2022, DOI:10.32604/biocell.2022.016718

Abstract To understand the molecular characteristics of the miR477 gene family of grape (Vvi-miR477) and to predict its target genes, the Vvi-miR477 genes were identified from previous small RNA sequencing data, then phylogenetic analysis and prediction of target gene were conducted. The Vvi-miR477 family consists of two precursor sequences and three mature sequences. The miR477 family members were mostly 19-22nt in length. The sequence is relatively conservative. Vvi-MIR477a and Vvi-MIR477b are located on chromosomes 1 and 2, respectively. These precursor sequences can form the typical stable stem-loop structure. Their minimum folding free energy is −39.10 kcal/mol and −50.90 kcal/mol, respectively. The… More >

PRIYANKA THAKUR¹, REENA V. SAINI^2,3,*, ANIL K. CHHILLAR⁴, NEERAJ K. SAINI⁵, VIJAY KUMAR THAKUR⁶, SAMARJEET SINGH SIWAL⁷, ADESH K. SAINI^2,3,*

BIOCELL, Vol.46, No.2, pp. 309-324, 2022, DOI:10.32604/biocell.2022.016916

Abstract Breast cancer, also recognized as the principal cause of cancer-related deaths among women, is the second most familiar and prevalent form of cancer. New diagnostic and prognostic biomarkers that are highly specific are urgently needed for its early prognosis. MicroRNAs (miRNAs), a class of non-coding RNAs, are known to control the biological processes involving transcription, post-transcriptional and covalent modifications, splicing, translation, cell differentiation, proliferation, apoptosis, cancer progression, and invasion. Any dysregulation in miRNA expression, demonstrating their oncogenic and tumor-suppressive functions, contributes to cancer progression. MicroRNA-21 (miR-21), an ‘onco-miR’ in breast cancer, is involved in tumor progression and metastasis by suppressing… More >

Dan Wang¹, Fengjuan Lu¹, Pengkai Wang¹, Jiaji Zhang¹, Lu Lu¹, Yan Zhen¹, Ye Lu¹, Tielong Cheng², Jisen Shi^1,*, Jinhui Chen^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.5, pp. 1445-1463, 2021, DOI:10.32604/phyton.2021.015499

Abstract Somatic cells respond to considerable stress, and go through a series of phytohormone pathways, then forming an embryo. The developmental process is recorded as somatic embryogenesis (SE). One of the key components regulating SE are the microRNAs (miRNAs). Despite previous studies, it is still not clear exactly how miRNAs exert their function of regulating targets during conditionally activated early SE. Here, we use Liriodendron sino-americanum as a model system and perform a combined analysis of microfluidic chips and degradome sequencing to study this process. We identified a total of 386 conserved miRNAs and 153 novel miRNAs during early SE. According… More >