Qiuying Quan*1,
Fengyun Zhong†1,
Xinwei Wang‡, Kai Chen§, Lingchuan Guo*
Oncology Research, Vol.27, No.7, pp. 779-788, 2019, DOI:10.3727/096504018X15442985680348
Abstract The aim of this study was to investigate the underlying mechanisms that transforming growth factor-
(TGF- )-mediated epithelial-to-mesenchymal transition (EMT) in tumor cells contributes to 5-FU resistance.
A series of experiments involving cell viability and caspase activity analyses, siRNA transfection, RNA isolation, and quantitative-PCR (qPCR) assay, cell migration analysis, Western blotting analysis of total protein and
membrane protein were performed in this study. Mouse xenograft model was used to determine the effect of
the PAR2 inhibitor in vivo. In this study, we found that protease-activated receptor 2 (PAR2) induction in 5-FU
therapy is correlated with TGF- -mediated EMT and apoptosis… More >
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