SHU ZHAO1,*, SHIXIN YE2
BIOCELL, Vol.47, No.6, pp. 1377-1388, 2023, DOI:10.32604/biocell.2023.023874
Abstract Background: This paper aims to establish a light-controlled phosphorylation detection method at the Y785 site
of tropomyosin receptor kinase A (TrkA) receptor in mammalian cells by using genetic code expansion technology and
detecting the effects of optical activation of this site on the downstream MAPK/ERK pathway. The study is based on the
current situation that the regulatory mechanism of TrkA phosphorylation has not been fully elucidated. Methods: Two
photosensitive unnatural amino acids, p-azido-L-phenylalanine (AzF) and photo-caged tyrosine (ONB) were introduced
into the TrkA-Y785 site by genetic code expansion technology and site-directed mutagenesis. Western blotting and laser
confocal imaging were conducted… More >
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