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    Assessment of protein quantification methods in Tetranychus urticae, as a potential tool for resistance detection to pesticides

    Cerna1 E, Y Ochoa2, R Mendoza1, MH Badii3, G Gallegos1, J Landeros1

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 147-152, 2010, DOI:10.32604/phyton.2010.79.147

    Abstract Protein assays were conducted on Tetranychus urticae Koch, as potential resistance detection tools to plaguicides. This is a phytophagous mite that feeds on a large variety of plants. Experiments were carried out using a pesticide susceptible and three field crop strains of T. urticae. Protein was measured by colorimetric assays, using Kit-II from Bio-Rad, with bovine serum albumin (BSA) as standard. Homogenates were prepared using 10, 30, 50, 100, 300, 500 and 800 mites, with 30 replicates each. Linearity was obtained for the standard curve of the different methods, and r2 values ranged from 0.877 to 0.985. The Bradford method… More >

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