Li G, FT Yang, XL Jiang, FX Chen, TH Cao, LC Wang

Phyton-International Journal of Experimental Botany, Vol.82, pp. 243-247, 2013, DOI:10.32604/phyton.2013.82.243

Abstract The aim of this study was to evaluate the impact of the plow pan on the production of spring corn. We evaluated SPAD values and chloroplast ultrastructure in premature ear leaves of spring maize at the grain-filling stage. The maize plants were grown in simulated plow pan or simulated subsoiling treatments. Plants in the simulated plow pan treatment showed irreversible damage to chloroplasts, including changes in chloroplast shape, disintegration and rupture of chloroplast membranes, and blurriness and cloudiness of grana lamellae. The chlorophyll content decreased, which is a typical characteristic of senescence. Subsoiling cultivation practices resulted in chloroplasts with a… More >

Marcelo de Castro Pazos*, Cristina Pacheco-Soares*, Newton Soares da Silva*, R.A. DaMatta**, M.T.T. Pacheco*

BIOCELL, Vol.27, No.3, pp. 301-309, 2003, DOI:10.32604/biocell.2003.27.301

Abstract The effects of Photodynamic Therapy using 2^nd generation photosensitizers have been widely investigated aiming clinical application treatment of solid neoplasms. In this work, ultrastructure changes caused by the action of two 2^nd generation photosensitizers and laser irradiation on CHO-K1 and HeLa (neoplastic) cells were analyzed by transmission electron microscopy. Aluminum phthalocyanine chloride, aluminum phthalocyanine tetrasulfonate chloride and radiation from a semiconductor laser at a fluency of 0.5 J/ cm² (Power=26mW; λ=670nm) were used. The results showed induction of apoptosis. Such alterations where observed in HeLa but not in CHO-K1 cells after Aluminum phthalocyanine tetrasulfonate chloride (AlPcS₄) photodynamic treatment. The Aluminum… More >

A. Farías, G.N. Hermida, L.E. Fiorito

BIOCELL, Vol.27, No.1, pp. 19-28, 2003, DOI:10.32604/biocell.2003.27.019

Abstract The ultrastructure of the intermediate segment (IS), distal tubule and collecting tubule (CT) of the south american toad Bufo arenarum, was studied by light and transmission electron microscopy. The IS is composed of cubical ciliated cells which propel the urine along the renal tubule. The distal tubule is divided into two portions: the early distal tubule (EDT) and the late distal tubule (LDT). The EDT is characterized by only one type of cells with well developed basolateral interdigitations and numerous elongated mitochondria, which are oriented normal to the basal surface. The “macula densa - like” is a specialized zone of… More >

GLADYS N. HERMIDA, ALEJANDRO FARÍAS, LUISA E. FIORITO

BIOCELL, Vol.26, No.3, pp. 347-355, 2002, DOI:10.32604/biocell.2002.26.347

Abstract The lung of the toad, Melanophryniscus stelzneri stelzneri was studied using scanning and transmission electron microscopy. In M.s.stelzneri the parenchyma forms a polygonal network arrangement, therefore the parenchyma is edicular. These spaces are delimited by the interconnection of third order septa which are covered by respiratory epithelium. Small patches of ciliated epithelium without goblet cells appear irregularly distributed on the septa. The respiratory epithelium consists of one type of pneumocyte, which shows characteristics of both type I and type II alveolar cells of higher vertebrates. The pneumocytes are irregular in shape and possess attenuated cytoplasmic processes, which spread around the… More >

ESSI KESKINEN¹ , YASUHARU TAKAKU² , V. BENNO MEYER-ROCHOW³, TAKAHIKO HARIYAMA⁴

BIOCELL, Vol.26, No.3, pp. 357-367, 2002, DOI:10.32604/biocell.2002.26.357

Abstract The aims of this paper have been (a) to characterize marginal ommatidia from different eye regions through a detailed description of their distinct ultrastructural features in three different size-classes of L. exotica, and (b) to compare microanatomical characteristics of the marginal ommatidia with those of ommatidia of the same eye, but located further centrally. On the basis of transverse as well as longitudinal sections we conclude that new ommatidia are added from a crescentic dorso-anterio-ventral edge of the eye and that maturing ommatidia go through a sequence in which originally the nuclei of cone -, pigment-, and retinula cells are… More >

DANIELA CARVALHO DOS SANTOS, ELISA APARECIDA GREGÓRIO

BIOCELL, Vol.26, No.2, pp. 229-235, 2002, DOI:10.32604/biocell.2002.26.229

Abstract The ultrastructure of the ovariole sheath along the Diatraea saccharalis ovariole was studied by scanning and transmission electron microscopy. Each ovariole is surrounded by an epithelial sheath, a tunica propria and scattered lumen cells. These three components of the ovariole sheath show different ultrastructural features along the ovariole, in the germarium or in the vitellarium; these differences are more evident in the epithelial sheath cells. The epithelial sheath is composed by two layers of cells, the external one running longitudinally and the internal one running circularly in the ovariole. These cells, in vitellarium, present cytoplasmic bundles of myofilaments that are… More >

LIDIA M. LOPEZ^*, AMANDA PELLEGRINO DE IRALDI^*, PATRICIA H. CARRIZO^**, MARTA DUBIN^**, ANDRÉS O.M. STOPPANI^**

BIOCELL, Vol.26, No.2, pp. 237-245, 2002, DOI:10.32604/biocell.2002.26.237

Abstract CG 10-248 (3,4-dihydro-2,2 dimethyl-9-chloro-2H-naphtho[1,2b]pyran-5,6-dione), a ß-lapachone analogue, modified the ultrastructure of rat liver mitochondria in vitro, in the absence of added oxidizable substrates. The condensed mitochondrial state was replaced by the orthodox or swollen state to a significant degree. The number of modified mitochondria depended on incubation time and quinone concentration, in the 25-100 µM range. Under the same experimental conditions, mitochondrial respiration was uncoupled as indicated by the increase in the rate of succinate oxidation by controlled mitochondria in metabolic state “4” (not in state “3”), and by the activation of latent F₀ F₁ -ATP synthase. Taking into account… More >

ARTURO D. JORGE^*, LUIS S. GUTIERREZ^**, OLIVER JORGE^*, MARIO H. BURGOS^**

BIOCELL, Vol.26, No.2, pp. 253-262, 2002, DOI:10.32604/biocell.2002.26.253

Abstract The aim of this paper is to establish whether there are cytochemical or ultrastructural alterations in the hepatocytes of patients with primary biliary cirrhosis (PBC) at stages I and II compared with the biopsies from individuals with normal liver. Cytochemical technique with ATP as substrate, transmission electron microscopy (TEM) and freeze fracture were used for the studies.
In the normal liver biopsies the ultrastructural cytochemical localization of the enzymatic activity was clearly shown in the bile canaliculi. In the PBC biopsies, the enzymatic activity is increased in the bile canaliculi and is also present in the lateral membranes of… More >

N.M.Y. Catalán, S.N. Fernández, B.C. Winik

BIOCELL, Vol.26, Suppl.S, pp. 91-100, 2002

Abstract This article has no abstract. More >

KHESLLER PATRÍCIA OLÁZIA NAME, GIULIANO PAGY FELIPE DOS REIS, SÔNIA NAIR BÁO

BIOCELL, Vol.31, No.2, pp. 229-236, 2007, DOI:10.32604/biocell.2007.31.229

Abstract The spermiogenesis of Sitophilus zeamais and Sitophilus oryzae, the maize and the rice weevil, respectively, was studied by light microscopy and scanning and transmission electron microscopy. Sitophilus spp. is the most widespread and destructive primary pest of stored cereals in the world. The spermiogenesis occurs within cysts. There are approximately 256 germ line cells per cyst. Inside each cysts, all the spermatids are in the same stage of maturation. The ultrastructure of the spermatozoa of S. zeamais and S. oryzae is similar to that described for other beetles. The head is formed by a three-layered acrosome with the perforatorium, the… More >