XUHE GONG1, GUANGPU FAN2, HUI LIU1, SIJIA WANG3, RONGCHONG HUANG1,*
BIOCELL, Vol.44, No.3, pp. 329-336, 2020, DOI:10.32604/biocell.2020.09639
Abstract This work aimed to study the beneficial role of human umbilical cord blood-derived mesenchymal stem cellconditioned medium (MSC-CM) in hypoxia-induced apoptosis in H9c2 cardiomyoblasts, in which the serine/heroine
kinases (Akt) pathway would be involved. For this, CM was collected by culturing MSCs in serum-free DMEM
medium for 24 h, and paracrine factors were analyzed by protein chip. H9c2 cells were divided into the following
groups: control group, hypoxia group, MSC-CM intervention group (CM group), MSC-CM + Akt phosphorylation
inhibitor (LY294002) group (LY group). Apoptosis of the H9c2 cells was tested with chromatin dye Hoechst 33342
and FITC-conjugated Annexin V apoptosis… More >
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