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  • Open Access


    A Simple Clear Technique in Observing Vascular Development of Grape Ovary

    Teng Fei1, Youmei Li1, Bo Li2, Zhaosen Xie1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2117-2132, 2023, DOI:10.32604/phyton.2023.028208

    Abstract The vascular system of the grapevine (Vitis vinifera L.) flower is a channel for transporting water and nutrients to the ovary. It plays an important role in the development of the ovary and fertilization through pollination. However, the vascular bundles in the flower are so tiny that they are difficult to sample and observe by traditional slicing techniques. In this study, ‘Summer Black’ grape flowers were selected as the test materials, and the tissue samples were treated by the optical clearing technique. After simple compaction, the structure and development of the vasculature were observed by common microscopy, fluorescence microscopy and… More >

  • Open Access


    Micropropagation of wild grapevines (Vitis spp.) of Central Mexico

    Jiménez-Martínez JH1, MG Gutiérrez-Martínez2, O Franco-Mora1, A González-Huerta2, AT Gutiérrez-Ibáñez2

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 107-112, 2013, DOI:10.32604/phyton.2013.82.107

    Abstract Two accessions of wild grapevines (Vitis spp.) native to Puebla State, Nealticán and Teziutlán, and other two native to Temascaltepec (1 and 2), Mexico State, were cultured in vitro using both Murashige-Skoog (MS) and woody plant medium (WPM) mediums. There were no differences between mediums for shoot generation. However, differences were shown among accessions for explant vigour, callus development, number of roots, leaves and nodes per explant. Rooting was evaluated only in MS medium by adding naphthalene acetic acid (NAA), indole butyric acid (IBA) or indole acetic acid (IAA) at 0.5 mg/L. With the exception of Temascaltepec 2, NAA generated… More >

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