Longwei Liu1, Praopim Limsakul1, Shaoying (Kathy) Lu1, Peter Yingxiao Wang1,*
Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 82-82, 2019, DOI:10.32604/mcb.2019.07360
Abstract Genetically-encoded biosensors based on Fluorescence Resonance Energy Transfer (FRET biosensors) have been widely used to dynamically track the activity of Protein Tyrosine Kinases (PTKs) in living cells because of their sensitive ratiometric fluorescence readout, high spatiotemporal resolution. However, the limitation in sensitivity, specificity, and dynamic range of these biosensors have hindered their broader applications, and there was a lack of efficient ways to optimize FRET biosensors. Here we established a rapid, systematic and universal approach for FRET biosensor optimization through directed evolution which involves generating genetic diversity and screening for protein variants with desired properties at the same time and… More >
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