Gonzalez-Franco AC1, L Robles-Hernandez1, A Nuñez-Barrios1, JL Strap2, DL Crawford3
Phyton-International Journal of Experimental Botany, Vol.78, pp. 83-90, 2009, DOI:10.32604/phyton.2009.78.083
Abstract In order to understand the temporal dynamics of actinomycete communities of the rhizosphere of the desert plant Artemisia tridentata (sagebrush), two complementary methods were used. They were: (1) 16S rDNA-based PCR coupled with denaturing gradient gel electrophoresis (DGGE), and (2) an agar plate enumeration methodology in which three different media were used to quantify total bacteria, actinomycetes, and fungi. The objective of this research were: (1) to obtain a comprehensive picture of the structure of actinomycete populations, and (2) their dynamics in the rhizosphere of young and old sagebrush plants during two distinct seasons. PCR-DGGE analysis showed that actinomycete groups… More >
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